Western Blot (WB)
(Western blot analysis of CD40/TNFRSF5 expression in mouse spleen extract (lane 1). CD40/TNFRSF5 at 40KD was detected using rabbit anti- CD40/TNFRSF5 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Rabbit anti-Mouse CD40/TNFRSF5 Polyclonal Antibody | anti-CD40 antibody
Anti-CD40/TNFRSF5 Antibody
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunohistochemistry(IHC) Paraffin: 0.5-1ug/ml
ELISA: 0.1-0.5ug/ml
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Western Blot (WB)
(Western blot analysis of CD40/TNFRSF5 expression in mouse spleen extract (lane 1). CD40/TNFRSF5 at 40KD was detected using rabbit anti- CD40/TNFRSF5 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Western Blot (WB)
(Western blot analysis of CD40/TNFRSF5 expression in mouse spleen extract (lane 1). CD40/TNFRSF5 at 40KD was detected using rabbit anti- CD40/TNFRSF5 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Immunohistochemistry (IHC)
(CD40/TNFRSF5 was detected in paraffin-embedded sections of mouse lymphaden tissues using rabbit anti- CD40/TNFRSF5 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method. )
Immunohistochemistry (IHC)
(CD40/TNFRSF5 was detected in paraffin-embedded sections of mouse lymphaden tissues using rabbit anti- CD40/TNFRSF5 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of U937 cells using anti-CD40/TNFRSF5 antibody (MBS178808).Overlay histogram showing U937 cells stained with MBS178808 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD40/TNFRSF5 Antibody (MBS178808,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight 488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of U937 cells using anti-CD40/TNFRSF5 antibody (MBS178808).Overlay histogram showing U937 cells stained with MBS178808 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD40/TNFRSF5 Antibody (MBS178808,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight 488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Background: CD40 is a cell surface receptor that is expressed on the surface of all mature B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. It is a member of the tumor necrosis factor (TNF) receptor superfamily and is the receptor for CD40 ligand. CD40 ligand (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.
2. Harding, S. A.; Sarma, J.; Josephs, D. H.; Cruden, N. L.; Din, J. N.; Twomey, P. J.; Fox, K. A. A.; Newby, D. E. : Upregulation of the CD40/CD40 ligand dyad and platelet-monocyte aggregation in cigarette smokers. Circulation 109: 1926-1929, 2004.
3. Kato, K.; Cantwell, M. J.; Sharma, S.; Kipps, T. J. : Gene transfer of CD40-ligand induces autologous immune recognition of chronic lymphocytic leukemia B cells. J. Clin. Invest. 101: 1133-1141, 1998.
4. Stamenkovic, I.; Clark, E. A.; Seed, B. : A B-lymphocyte activation molecule related to the nerve growth factor receptor and induced by cytokines in carcinomas. EMBO J. 8: 1403-1410, 1989.
5. van Kooten, C.; Banchereau, J. : CD40-CD40 ligand. J. Leuko. Biol. 67: 2-17, 2000.
NCBI and Uniprot Product Information
Uniprot Description
CD40: a member of the TNF-receptor superfamily. This receptor for CD40L mediates a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. Defects in CD40 are the cause of hyper-IgM immunodeficiency type 3 (HIGM3). HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. Two alternatively spliced isoforms have been reported. Isoform I is a type I membrane protein; isoform II is secreted.
Protein type: Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: 2 H3|2 85.38 cM
Cellular Component: cell soma; cell surface; cytoplasm; external side of plasma membrane; extracellular space; intracellular membrane-bound organelle; plasma membrane
Molecular Function: antigen binding; enzyme binding; protein binding; protein domain specific binding; tumor necrosis factor receptor activity; ubiquitin protein ligase binding
Biological Process: activation of NF-kappaB transcription factor; B cell activation; cellular calcium ion homeostasis; defense response to protozoan; defense response to virus; immune response-regulating cell surface receptor signaling pathway; inflammatory response; positive regulation of B cell proliferation; positive regulation of GTPase activity; positive regulation of interleukin-12 production; positive regulation of isotype switching to IgG isotypes; positive regulation of MAP kinase activity; positive regulation of MAPKKK cascade; positive regulation of protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat1 protein; protein kinase B signaling cascade; regulation of apoptosis; regulation of immune response; regulation of immunoglobulin secretion; response to lipopolysaccharide
Research Articles on CD40
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Product Notes
The CD40 cd40 (Catalog #AAA178808) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD40/TNFRSF5 Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD40/TNFRSF5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA). Western Blot: 0.1-0.5ug/ml Immunohistochemistry(IHC) Paraffin: 0.5-1ug/ml ELISA: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the CD40 cd40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD40/TNFRSF5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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