Mouse Chemokine C-C-Motif Receptor 1 (CCR1) ELISA Kit | CCR1 elisa kit

Mouse Chemokine C-C-Motif Receptor 1 (CCR1) ELISA Kit

Cat. Num. AAA2020135

• Gene Names: CCR1; CKR1; CD191; CKR-1; HM145; CMKBR1; MIP1aR; SCYAR1
• Reactivity: Mouse
• Synonyms: Chemokine C-C-Motif Receptor 1 (CCR1); Mouse Chemokine C-C-Motif Receptor 1 (CCR1) ELISA Kit; CD191; CMKBR1; HM145; MIP1aR; SCYAR1; RANTES-R; Macrophage inflammatory protein 1-alpha receptor; LD78 receptor; CCR1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Chemokine C-C-Motif Receptor 1 (CCR1).; No significant cross-reactivity or interference between Chemokine C-C-Motif Receptor 1 (CCR1) and analogues was observed.
• Sequence Length: 355

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-C-Motif Receptor 1 (CCR1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-C-Motif Receptor 1 (CCR1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCR1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Receptor 1 (CCR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-C-Motif Receptor 1 (CCR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-C-Motif Receptor 1 (CCR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-C-Motif Receptor 1 (CCR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CCR1 elisa kit

Signal transduction; CD & Adhesion molecule; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 40787801
• NCBI GeneID: 1230
• UniProt Accession #: P32246; Q86VA9
• Molecular Weight: 41,173 Da
• NCBI Official Full Name: Chemokine (C-C motif) receptor 1
• NCBI Official Synonym Full Names: chemokine (C-C motif) receptor 1
• NCBI Official Symbol: CCR1
• NCBI Official Synonym Symbols: CKR1; CD191; CKR-1; HM145; CMKBR1; MIP1aR; SCYAR1
• NCBI Protein Information: C-C chemokine receptor type 1
• UniProt Protein Name: C-C chemokine receptor type 1
• Protein Family: C-C chemokine receptor
• UniProt Gene Name: CCR1
• UniProt Synonym Gene Names: CMKBR1; CMKR1; SCYAR1; C-C CKR-1; CC-CKR-1; CCR-1; CCR1; MIP-1alpha-R
• UniProt Entry Name: CCR1_HUMAN

NCBI Description

This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The ligands of this receptor include macrophage inflammatory protein 1 alpha (MIP-1 alpha), regulated on activation normal T expressed and secreted protein (RANTES), monocyte chemoattractant protein 3 (MCP-3), and myeloid progenitor inhibitory factor-1 (MPIF-1). Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. Knockout studies of the mouse homolog suggested the roles of this gene in host protection from inflammatory response, and susceptibility to virus and parasite. This gene and other chemokine receptor genes, including CCR2, CCRL2, CCR3, CCR5 and CCXCR1, are found to form a gene cluster on chromosome 3p. [provided by RefSeq, Jul 2008]

Uniprot Description

CCR1: Receptor for a C-C type chemokine. Binds to MIP-1-alpha, MIP-1-delta, RANTES, and MCP-3 and, less efficiently, to MIP-1- beta or MCP-1 and subsequently transduces a signal by increasing the intracellular calcium ions level. Responsible for affecting stem cell proliferation. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; C-C chemokine receptor activity; chemokine receptor activity; C-C chemokine binding; phosphoinositide phospholipase C activity

Biological Process: exocytosis; positive regulation of osteoclast differentiation; metabolic process; cytokine and chemokine mediated signaling pathway; chemotaxis; cellular calcium ion homeostasis; negative regulation of bone mineralization; G-protein signaling, coupled to cyclic nucleotide second messenger; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; cell-cell signaling; calcium ion transport; response to wounding; dendritic cell chemotaxis; immune response; inflammatory response; positive regulation of calcium ion transport; cell adhesion