Rabbit anti-Human CD-14 Polyclonal Antibody | anti-CD-14 antibody

CD-14 Antibody (NT)

Cat. Num. AAA840451

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Synonyms: CD-14; Polyclonal Antibody; CD-14 Antibody (NT); CD14; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD_antigen=CD14; urinary form; membrane-bound form.; anti-CD-14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Form/Format: 100 ul of antibody in PBS with 0.09% (W/V) sodium azide; Appearance: Colorless liquid
• Sequence Length: 375

• Applicable Applications for anti-CD-14 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Western blot: ~1:1000, IHC: ~1:10-1:50, IF: ~1:10-1:50, FACS: ~1:10-1:50.
• Immunogen: This CD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human CD14.
• Positive Control: Western blot: 293 cell lysate, IHC: human lung carcinoma, FACS: A549 cells, IF: A549 cells
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western blot

(Western blot analysis of CD14 (arrow) using rabbit polyclonal CD14 Antibody (NT). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2) and A549 cell line lysates (35 ug/lane) used for analysis.)

Flowcytometry

(FACS analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies was used for the analysis.)

Immunohistochemistry

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD14 antibody (N-term) (Cat # MBS840451), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Imunofluorescence

(Confocal IF analysis of CD14 Antibody (N-term) (Cat # MBS840451) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-CD-14 antibody

Polyclonal Antibody to detect CD-14 in human samples

Background: Lipopolysaccharide (LPS) elicits the secretion of mediators and cytokines produced by activated macrophages and monocytes. CD14 is a glycosylphosphatidylinositol (GPI)-anchored protein found on the surfaces of monocytes and polymorphonuclear leukocytes. CD14 functions as a receptor for LPS, resulting in the secretion of various proteins. An important component in the LPS activation of monocytes through the CD14 receptor is the "adapter molecule," lipopolysaccharide binding protein (LBP). There are two forms of CD14, a membrane-associated form (mCD14), and a soluble form (sCD14). mCD14 responds to LPS alone and facilitates the secretion of proteins, while cells not expressing mCD14 fail to respond to LPS. The cells that lack mCD14 respond to LPS/LBP in the presence of sCD14.

Product Categories/Family for anti-CD-14 antibody

Antibodies & Supporting Tools; Antibodies for Alzheimer's Research Neuroscience; AntibodiesCytokines; Growth Factors & Hormones; Antibodies to Cytokines & Growth Factors (A-Z) Antibodies & Supporting Tools; Primary Antibodies (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 4557417
• NCBI GeneID: 929
• NCBI Accession #: NP_000582.1
• NCBI GenBank Nucleotide #: NM_000591.3
• UniProt Accession #: P08571; Q53XT5; Q96FR6; Q96L99; Q9UNS3
• Molecular Weight: ~40.07 kDa
• NCBI Official Full Name: monocyte differentiation antigen CD14
• NCBI Official Synonym Full Names: CD14 molecule
• NCBI Official Symbol: CD14
• NCBI Protein Information: monocyte differentiation antigen CD14
• UniProt Protein Name: Monocyte differentiation antigen CD14
• Protein Family: Cd-146 peptide
• UniProt Gene Name: CD14
• UniProt Entry Name: CD14_HUMAN

NCBI Description

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]

Uniprot Description

CD14: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; cell surface; extracellular region; plasma membrane; endosome membrane; lipid raft

Molecular Function: peptidoglycan receptor activity; protein binding; opsonin receptor activity; lipopolysaccharide binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; apoptosis; MyD88-independent toll-like receptor signaling pathway; positive regulation of endocytosis; toll-like receptor 3 signaling pathway; positive regulation of tumor necrosis factor production; phagocytosis; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to magnesium ion; cell surface receptor linked signal transduction; response to ethanol; response to heat; toll-like receptor signaling pathway; innate immune response; inflammatory response; response to electrical stimulus; toll-like receptor 4 signaling pathway; positive regulation of cytokine secretion