Mouse anti-Human CD14 Monoclonal Antibody | anti-CD14 antibody

CD14 Monoclonal Antibody

Cat. Num. AAA9415034

• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein G Purifed
• Synonyms: CD14; Monoclonal Antibody; CD14 Monoclonal Antibody; CD14 molecule; anti-CD14 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Specificity: Specific for Recombinant CD14 Protein denatured and native forms
• Purity/Purification: Protein G Purifed
• Form/Format: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 375

• Applicable Applications for anti-CD14 antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:20 - 1:200
• Immunogen: Recombinant CD14 Protein
• Immunogen Type: Protein
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human lung using in 30ug/ml dilute concentrations.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human testis using in 30ug/ml dilute concentrations..)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human kidney using in 30ug/ml dilute concentrations.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human lung using in 30ug/ml dilute concentrations.)

Related Product Information for anti-CD14 antibody

As a component of the innate immune system, the cell surface glycoprotein CD14 is a myelomonocytic differentiation antigen preferentially expressed on monocytes, macrophages, and activated granulocytes. CD14 exists as two forms, either anchored into the membrane by a GPI-anchor tail (mCD14) or present as a soluble form (sCD14) in normal serum and body fluids. CD14 was first described as a pattern recognition receptor for lipopolysaccharide (LPS) and a variety of ligands derived from different microbial sources, along with the co-receptors Toll-like receptor TLR 4 and MD-2. The binding of CD14 and LPS depends on the presence and catalytic activity of lipopolysaccharide-binding protein (LBP). Besides its endotoxin signaling function, CD14 has been proposed to be involved in various biological processes, including transportation of other lipids, cell-cell interaction during different immune responses, as well as recognition of apoptotic cells.Multiple transcript variants resulting from alternative splicing encode the same isoform of CD14.

Product Categories/Family for anti-CD14 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4557417
• NCBI GeneID: 929
• NCBI Accession #: NP_000582.1
• NCBI GenBank Nucleotide #: NM_000591.3
• UniProt Accession #: P08571; Q53XT5; Q96FR6; Q96L99; Q9UNS3
• Molecular Weight: 40,076 Da
• NCBI Official Full Name: monocyte differentiation antigen CD14
• NCBI Official Synonym Full Names: CD14 molecule
• NCBI Official Symbol: CD14
• NCBI Protein Information: monocyte differentiation antigen CD14
• UniProt Protein Name: Monocyte differentiation antigen CD14
• Protein Family: Monocyte differentiation antigen
• UniProt Gene Name: CD14
• UniProt Entry Name: CD14_HUMAN

Uniprot Description

CD14: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: anchored to external side of plasma membrane; endosome membrane; extracellular region; extracellular space; Golgi apparatus; lipid raft; lipopolysaccharide receptor complex; plasma membrane

Molecular Function: lipopolysaccharide binding; opsonin receptor activity; peptidoglycan receptor activity; protein binding

Biological Process: apoptosis; cell surface receptor linked signal transduction; I-kappaB kinase/NF-kappaB cascade; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of MyD88-independent toll-like receptor signaling pathway; phagocytosis; positive regulation of interferon-gamma production; positive regulation of tumor necrosis factor production; toll-like receptor 4 signaling pathway