Rabbit anti-Human Protein Kinase D2 Polyclonal Antibody | anti-PKD2 antibody

Protein Kinase D2, CT (Serine/Threonine-protein Kinase D2, nPKC-D2, PRKD2, PKD2, HSPC187) (Azide free) (HRP)

Cat. Num. AAA6495294

• Gene Names: PRKD2; PKD2; HSPC187; nPKC-D2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: Protein Kinase D2; Polyclonal Antibody; CT (Serine/Threonine-protein Kinase D2; nPKC-D2; PRKD2; PKD2; HSPC187) (Azide free) (HRP); anti-PKD2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Protein Kinase D2.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PKD2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 937-968 in the C-terminal region of human Protein Kinase D2, UniProt Accession #Q13563.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis to detect PKD2 in HL-60 cell lysate using P3103-73J3.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue reacted with P3103-73J3, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of P3103-73J3 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-PKD2 antibody

PKD2, a member of the polycystin family, functions as a calcium permeable cation channel. PKD1 and PKD2 may function through a common signaling pathway that is necessary for normal tubulogenesis. PKD2 interacts with PKD1, potentially through the C-terminal region. PKD1 requires the presence of PKD2 for stable expression. PKD2 also interacts with CD2AP. This protein is strongly expressed in ovary, fetal and adult kidney, testis, and small intestine, but is not detected in peripheral leukocytes. Defects in PKD2 are the cause of autosomal dominant polycystic kidney disease type II (ADPKD-2) which represent approximately 15% of cases of autosomal dominant polycystic kidney disease, a common autosomal dominant genetic disease affecting about 1 out 1000 individuals. ADPKD is characterized by progressive formation and enlargement of cysts in both kidneys, typically leading to end-stage renal disease in adult life. Cysts also occurs in the liver and other organs. All mutations, scattered between exons 1 and 11, result in a truncated PKD2 that lacks both the calcium-binding EF-hand domain and the two cytoplasmic domains required for the interaction of PKD2 with PKD1 and with itself. ADPKD type II is clinically milder than ADPKD type I, but it has a deleterious impact on overall life expectancy.

Product Categories/Family for anti-PKD2 antibody

Antibodies; Protein Kinases

References

1. Stayner, C., et al., Trends Pharmacol. Sci. 22(11):543-546 (2001). 2. Lehtonen, S., et al., J. Biol. Chem. 275(42):32888-32893 (2000). 3. Watnick, T., et al., Nat. Genet. 25(2):143-144 (2000). 4. Torra, R., et al., Kidney Int. 56(1):28-33 (1999). 5. Hayashi, T., et al., Genomics 44(1):131-136 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 120659782
• NCBI GeneID: 25865
• NCBI Accession #: NP_001073349.1
• NCBI GenBank Nucleotide #: NM_001079880.1
• UniProt Accession #: Q9BZL6; Q13563
• Molecular Weight: 96,750 Da
• NCBI Official Full Name: serine/threonine-protein kinase D2 isoform A
• NCBI Official Synonym Full Names: protein kinase D2
• NCBI Official Symbol: PRKD2
• NCBI Official Synonym Symbols: PKD2; HSPC187; nPKC-D2
• NCBI Protein Information: serine/threonine-protein kinase D2
• UniProt Protein Name: Serine/threonine-protein kinase D2
• Protein Family: Polycystin
• UniProt Gene Name: PRKD2
• UniProt Synonym Gene Names: PKD2
• UniProt Entry Name: KPCD2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PRKD2: a calcium-independent, phospholipid-dependent CAMK kinase of the PKD family. Activated by diacylglycerol and phorbol esters. may play a role in carcinoid tumor progression.

Protein type: Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.13; Motility/polarity/chemotaxis; Kinase, protein; CAMK group; PKD family

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; plasma membrane; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; metal ion binding; ATP binding; protein kinase activity

Biological Process: cell death; positive regulation of cell adhesion; protein amino acid autophosphorylation; positive regulation of blood vessel endothelial cell migration; activation of CREB transcription factor; positive regulation of fibroblast growth factor receptor signaling pathway; protein amino acid phosphorylation; positive regulation of peptidyl-serine phosphorylation; T cell receptor signaling pathway; positive regulation of vascular endothelial growth factor receptor signaling pathway; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; positive regulation of angiogenesis; peptidyl-serine phosphorylation; positive regulation of T cell receptor signaling pathway; positive regulation of interleukin-2 production; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; angiogenesis; vascular endothelial growth factor receptor signaling pathway; cell adhesion