Rabbit anti-Human Isocitrate Dehydrogenase Polyclonal Antibody | anti-ICDH antibody

Isocitrate Dehydrogenase, CT (Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial [Precursor], Isocitric dehydrogenase, NAD(+)-specific ICDH, IDH3A, IDH3)

Cat. Num. AAA624120

• Gene Names: ICDH; F20D21.16; F20D21_16; ICDH; isocitrate dehydrogenase
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: Isocitrate Dehydrogenase; Polyclonal Antibody; CT (Isocitrate dehydrogenase [NAD] subunit alpha; mitochondrial [Precursor]; Isocitric dehydrogenase; NAD(+)-specific ICDH; IDH3A; IDH3); Anti -Isocitrate Dehydrogenase; anti-ICDH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IDH3.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-ICDH antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500
• Immunogen: Synthetic peptide selected from the C-terminal region of human Isocitrate dehydrogenase (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Isocitrate dehydrogenase in MCF-7, Jurkat cell line lysates (35ug/lane). Isocitrate dehydrogenase (arrow) was detected using the purified Pab.)

Related Product Information for anti-ICDH antibody

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein described here is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.

Product Categories/Family for anti-ICDH antibody

Antibodies; Abs to Enzymes; Dehydrogenase

NCBI and Uniprot Product Information

• NCBI GI #: 15221788
• NCBI GeneID: 841875
• NCBI Accession #: NP_175836.1
• NCBI GenBank Nucleotide #: NM_104312.2
• UniProt Accession #: Q9SLK0
• Molecular Weight: 47,234 Da
• NCBI Official Full Name: isocitrate dehydrogenase
• NCBI Official Symbol: ICDH
• NCBI Official Synonym Symbols: F20D21.16; F20D21_16; ICDH; isocitrate dehydrogenase
• NCBI Protein Information: isocitrate dehydrogenase
• UniProt Protein Name: Isocitrate dehydrogenase [NADP]
• Protein Family: Isocitrate dehydrogenase
• UniProt Gene Name: F20D21.16
• UniProt Synonym Gene Names: ICDH
• UniProt Entry Name: Q9SLK0_ARATH

NCBI Description

NADP-specific isocitrate dehydrogenase (ICDH)

Uniprot Description

Catalytic activity: Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. PIRNR PIRNR000108Oxalosuccinate + NADP+ = 2-oxoglutarate + CO2 + NADPH. PIRNR PIRNR000108

Cofactor: Binds 1 magnesium or manganese ion per subunit

By similarity. PIRNR PIRNR000108 PIRSR PIRSR000108-3

Sequence similarities: Belongs to the isocitrate and isopropylmalate dehydrogenases family. PIRNR PIRNR000108