Rabbit CCT5 Polyclonal Antibody | anti-CCT5 antibody

CCT5 antibody - N-terminal region

Cat. Num. AAA3211884

• Gene Names: CCT5; CCTE; HEL-S-69; PNAS-102; CCT-epsilon; TCP-1-epsilon
• Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified
• Synonyms: CCT5; Polyclonal Antibody; CCT5 antibody - N-terminal region; anti-CCT5 antibody

• Host: Rabbit
• Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
• Clonality: Polyclonal
• Purity/Purification: Affinity Purified
• Form/Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Sequence: Synthetic peptide located within the following region: NDGATILSMMDVDHQIAKLMVELSKSQDDEIGDGTTGVVVLAGALLEEAE
• Sequence Length: 541

• Applicable Applications for anti-CCT5 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 100%; Zebrafish: 100%
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CCT5
• Preparation and Storage: For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Rabbit Anti-CCT5 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human heart TissueObserved Staining: CytoplasmicPrimary Antibody Concentration: 1:100Other Working Concentrations: N/ASecondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)

Western Blot (WB)

(Lanes:1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug)Primary Antibody Dilution:2ug/mlSecondary Antibody:IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary Antibody Dilution:1: 20,000Gene Name:CCT5Submitted by:Dr. Yuzhi Chen, University of Arkansas for Medical ScienceCCT5 is supported by BioGPS gene expression data to be expressed in HEK293)

Western Blot (WB)

(Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical ScienceApplication: Western blottingSpecies+tissue/cell type: Mouse WT brain and Rat brainHow many ug's of tissue/cell lysate run on the gel: 1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug)Primary antibody dilution: 2ug/mlSecondary antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary antibody dilution: 1: 20,000)

Western Blot (WB)

(WB Suggested Anti-CCT5 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Human brain)

Related Product Information for anti-CCT5 antibody

This is a rabbit polyclonal antibody against CCT5. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: CCT5 is a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Product Categories/Family for anti-CCT5 antibody

Polyclonal; Drugs and Drug Metabolism; Various

NCBI and Uniprot Product Information

• NCBI GI #: 24307939
• NCBI GeneID: 22948
• NCBI Accession #: NP_036205
• NCBI GenBank Nucleotide #: NM_012073
• UniProt Accession #: P48643
• Molecular Weight: 60kDa
• NCBI Official Full Name: T-complex protein 1 subunit epsilon isoform a
• NCBI Official Synonym Full Names: chaperonin containing TCP1 subunit 5
• NCBI Official Symbol: CCT5
• NCBI Official Synonym Symbols: CCTE; HEL-S-69; PNAS-102; CCT-epsilon; TCP-1-epsilon
• NCBI Protein Information: T-complex protein 1 subunit epsilon
• UniProt Protein Name: T-complex protein 1 subunit epsilon
• Protein Family: T-complex protein
• UniProt Gene Name: CCT5
• UniProt Synonym Gene Names: CCTE; KIAA0098; TCP-1-epsilon
• UniProt Entry Name: TCPE_HUMAN

NCBI Description

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Mutations in this gene cause hereditary sensory and autonomic neuropathy with spastic paraplegia (HSNSP). Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 13. [provided by RefSeq, Apr 2015]

Uniprot Description

CCT-epsilon: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Defects in CCT5 are the cause of hereditary sensory neuropathy autosomal recessive with spastic paraplegia (HSNSP). The disease is characterized by spastic paraplegia and progressive distal sensory neuropathy leading to mutilating ulcerations of the upper and lower limbs. Belongs to the TCP-1 chaperonin family.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 5p15.2

Cellular Component: chaperonin-containing T-complex; microtubule; centrosome; cytoplasm; nucleolus; cytosol

Molecular Function: protein binding; unfolded protein binding; beta-tubulin binding; G-protein beta-subunit binding; ATP binding

Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; binding of sperm to zona pellucida; response to virus

Disease: Neuropathy, Hereditary Sensory, With Spastic Paraplegia, Autosomal Recessive