Rabbit Septin 7 Polyclonal Antibody | anti-M368 antibody

Septin 7 (M368) Polyclonal Antibody

Cat. Num. AAA3004467

• Gene Names: SEPTIN7; CDC3; CDC10; SEPT7; SEPT7A; NBLA02942
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Septin 7; Polyclonal Antibody; Septin 7 (M368) Polyclonal Antibody; Septin-7; CDC10 protein homolog; SEPT7; CDC10; anti-M368 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Septin 7 (M368) polyclonal antibody detects endogenous levels of Septin 7 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 437

• Applicable Applications for anti-M368 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 332-389 of Human Septin 7.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Septin 7 (M368) pAb at 1:500 dilutionLane1:Hela whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:The Brain tissue lysate of Mouse (40ug) Lane4:The Brain tissue lysate of Rat (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Septin 7 (M368) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.)

Related Product Information for anti-M368 antibody

The septins are a family of GTPase enzymes, some of which are required for cytokinesis and others of which are associated with exocytosis. Members of the septin family can form heteropolymer complexes and also play a role in the organization of new growth in organisms. The transcriptional regulation of all septins is complex, resulting in alternatively spliced variants. At least three septins (Septin 1, 2 and 4) are associated with a Tau-based paired helical filament core and may contribute to the formation of neurofibrillary tangle as integral constituents of paired helical filaments. Septin 3 (G-Septin), a GTP-binding protein, is highly expressed in brain and is regulated by protein kinase G in neurons.

NCBI and Uniprot Product Information

• NCBI GI #: 148352331
• NCBI GeneID: 989
• NCBI Accession #: NP_001779.3
• NCBI GenBank Nucleotide #: NP_001779.3
• UniProt Accession #: Q16181
• Molecular Weight: ~ 51kDa
• NCBI Official Full Name: septin-7 isoform 1
• NCBI Official Synonym Full Names: septin 7
• NCBI Official Symbol: SEPTIN7
• NCBI Official Synonym Symbols: CDC3; CDC10; SEPT7; SEPT7A; NBLA02942
• NCBI Protein Information: septin-7
• UniProt Protein Name: Septin-7
• UniProt Gene Name: SEPT7
• UniProt Synonym Gene Names: CDC10
• UniProt Entry Name: SEPT7_HUMAN

NCBI Description

This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. [provided by RefSeq, Jul 2011]

Uniprot Description

SEPT7: Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements. Belongs to the septin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Hydrolase; Cytoskeletal

Chromosomal Location of Human Ortholog: 7p14.2

Cellular Component: cell cortex; actin cytoskeleton; microtubule cytoskeleton; cytoplasm; apical plasma membrane; plasma membrane; nucleolus; stress fiber; spindle; synapse; midbody; axoneme; nerve terminal; nucleus; cleavage furrow

Molecular Function: identical protein binding; protein binding; GTP binding; structural molecule activity

Biological Process: mitosis; protein heterooligomerization; cytokinesis; shape changes of embryonic cells