Typical Testing Data/Standard Curve (for reference only)
Monkey Angiopoietin Like Protein 3 ELISA Kit | ANGPTL3 elisa kit
Monkey Angiopoietin Like Protein 3 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This ANGPTL3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey ANGPTL3. Th is ELISA kit for research use only!
PRINCIPLE OF THE ASSAY ANGPTL3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ANGPTL3. Standards or samples are then added to the microtiter plate wells and ANGPTL3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ANGPTL3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ANGPTL3 are added to each well to “sandwich” the ANGPTL3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ANGPTL3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANGPTL3 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of a family of secreted proteins that function in angiogenesis. The encoded protein, which is expressed predominantly in the liver, is further processed into an N-terminal coiled-coil domain-containing chain and a C-terminal fibrinogen chain. The N-terminal chain is important for lipid metablism, while the C-terminal chain may be involved in angiogenesis. Mutations in this gene cause familial hypobetalipoproteinemia type 2. [provided by RefSeq, Feb 2013]
Uniprot Description
ANGPTL3: Defects in ANGPTL3 are the cause of familial hypobetalipoproteinemia type 2 (FHBL2); also called combined hypobetalipoproteinemia familial. FHBL2 is a disorder of lipid metabolism characterized by less than 5th percentile age- and sex-specific levels of low density lipoproteins, and dietary fat malabsorption. Affected individuals present with combined hypolipidemia, consisting of extremely low plasma levels of LDL cholesterol, HDL cholesterol, and triglycerides.
Protein type: Inhibitor; Secreted; Secreted, signal peptide; Cell adhesion; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 1p31.3
Cellular Component: extracellular space; cell surface
Molecular Function: integrin binding; enzyme inhibitor activity; phospholipase inhibitor activity; growth factor activity
Biological Process: integrin-mediated signaling pathway; cholesterol metabolic process; glycerol metabolic process; phospholipid catabolic process; cell-matrix adhesion; lipid homeostasis; positive regulation of lipid catabolic process; fatty acid metabolic process; signal transduction; sequestering of lipid; cholesterol homeostasis; positive regulation of angiogenesis; acylglycerol homeostasis; phospholipid metabolic process; artery morphogenesis; negative regulation of lipoprotein lipase activity; phospholipid homeostasis; positive regulation of cell migration
Disease: Hypobetalipoproteinemia, Familial, 2
Research Articles on ANGPTL3
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Product Notes
The Monkey ANGPTL3 angptl3 (Catalog #AAA739163) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA739163 ELISA Kit recognizes Monkey ANGPTL3. It is sometimes possible for the material contained within the vial of "Angiopoietin Like Protein 3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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