Rabbit anti-Mouse, Human CCT5 / TCP1 Epsilon Polyclonal Antibody | anti-CCT5 antibody

Anti-CCT5 / TCP1 Epsilon Antibody (aa89-338) IHC-plus

Cat. Num. AAA2400153

• Gene Names: CCT5; CCTE; HEL-S-69; PNAS-102; CCT-epsilon; TCP-1-epsilon
• Reactivity: Mouse, Human
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot
• Purity: Immunoaffinity purified
• Synonyms: CCT5 / TCP1 Epsilon; Polyclonal Antibody; Anti-CCT5 / TCP1 Epsilon Antibody (aa89-338) IHC-plus; Rabbit Polyclonal (IgG) to Human CCT5 / TCP1 Epsilon; Human CCT5 / TCP1 Epsilon; KIAA0098; CCT-epsilon; CCTE; TCP-1-epsilon; TCP1 Epsilon; anti-CCT5 antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human CCT5 / TCP1 Epsilon
• Purity/Purification: Immunoaffinity purified
• Form/Format: 0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 541

• Applicable Applications for anti-CCT5 antibody: Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: ICC (1:100 - 1:1000); IHC-P (7.5 ug/ml); WB (1:1000 - 1:10000)
• Immunogen: CCT5 / TCP1 Epsilon antibody was raised against recombinant fragment corresponding to a region within amino acids 89 and 338 of TCP1 epsilon (SwissProt P48643). Percent identity by BLAST analysis: Human (100%); Bovine (95%); Rat (94%); Chicken (88%); Xenopus (86%); Zebrafish (84%).
• Immunogen Description: Recombinant fragment corresponding to a region within amino acids 89 and 338 of TCP1 epsilon (SwissProt P48643). Percent identity by BLAST analysis: Human (100%); Bovine (95%); Rat (94%); Chicken (88%); Xenopus (86%); Zebrafish (84%).
• Immunogen Type: Recombinant protein
• Antigen Modification: aa89-338
• Target Species: Human
• Preparation and Storage: Keep as concentrated solution. Aliquot and store at -20 degree C or below. Avoid multiple freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-CCT5 / TCP1 Epsilon antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.)

Immunocytochemistry (ICC)

(Immunofluorescence of paraformaldehyde-fixed HeLa using TCP-1 epsilon antibody at 1:200 dilution.)

Western Blot (WB)

(Sample (30 ug of whole cell lysate). A: Molt-4. 7.5% SDS PAGE. CCT5 / TCP1 Epsilon antibody diluted at 1:1000)

Western Blot (WB)

(Sample (50 ug of whole cell lysate). A: mouse brain. 7.5% SDS PAGE. CCT5 / TCP1 Epsilon antibody diluted at 1:10000.)

Related Product Information for anti-CCT5 antibody

CCT5 Antibody, KIAA0098 Antibody, CCT-epsilon Antibody, CCTE Antibody, TCP-1-epsilon Antibody, TCP1 Epsilon Antibody Description: CCT5 / TCP1 Epsilon is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin.

NCBI and Uniprot Product Information

• NCBI GI #: 1351211
• NCBI GeneID: 22948
• UniProt Accession #: P48643; A8JZY8; A8K2X8; B4DYD8
• Molecular Weight: 49,526 Da
• NCBI Official Full Name: T-complex protein 1 subunit epsilon
• NCBI Official Synonym Full Names: chaperonin containing TCP1 subunit 5
• NCBI Official Symbol: CCT5
• NCBI Official Synonym Symbols: CCTE; HEL-S-69; PNAS-102; CCT-epsilon; TCP-1-epsilon
• NCBI Protein Information: T-complex protein 1 subunit epsilon
• UniProt Protein Name: T-complex protein 1 subunit epsilon
• Protein Family: T-complex protein
• UniProt Gene Name: CCT5
• UniProt Synonym Gene Names: CCTE; KIAA0098; TCP-1-epsilon
• UniProt Entry Name: TCPE_HUMAN

NCBI Description

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Mutations in this gene cause hereditary sensory and autonomic neuropathy with spastic paraplegia (HSNSP). Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 13. [provided by RefSeq, Apr 2015]

Uniprot Description

CCT-epsilon: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Defects in CCT5 are the cause of hereditary sensory neuropathy autosomal recessive with spastic paraplegia (HSNSP). The disease is characterized by spastic paraplegia and progressive distal sensory neuropathy leading to mutilating ulcerations of the upper and lower limbs. Belongs to the TCP-1 chaperonin family.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 5p15.2

Cellular Component: centrosome; chaperonin-containing T-complex; cytoplasm; cytosol; microtubule; myelin sheath; nucleolus

Molecular Function: ATP binding; beta-tubulin binding; G-protein beta-subunit binding; protein binding; unfolded protein binding

Biological Process: 'de novo' posttranslational protein folding; binding of sperm to zona pellucida; cellular protein metabolic process; positive regulation of telomere maintenance via telomerase; protein folding; protein stabilization; response to virus

Disease: Neuropathy, Hereditary Sensory, With Spastic Paraplegia, Autosomal Recessive