Rabbit CCR4-NOT transcription complex subunit 4 Polyclonal Antibody | anti-CNOT4 antibody

CCR4-NOT transcription complex subunit 4 (CNOT4, CCR4-associated factor 4, CLONE243, E3 ubiquitin protein ligase CNOT4, NOT4, NOT4H, Potential transcriptional repressor NOT4Hp)

Cat. Num. AAA619455

• Gene Names: CNOT4; NOT4; NOT4H; CLONE243
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: CCR4-NOT transcription complex subunit 4; Polyclonal Antibody; CCR4-NOT transcription complex subunit 4 (CNOT4; CCR4-associated factor 4; CLONE243; E3 ubiquitin protein ligase CNOT4; NOT4; NOT4H; Potential transcriptional repressor NOT4Hp); Anti -CCR4-NOT transcription complex subunit 4 (CNOT4; anti-CNOT4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human CNOT4. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-CNOT4 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1-2ug/ml
• Immunogen: Synthetic peptide corresponding to 19aa from near the amino terminus of the human CNOT4.
• Positive Control: A549 Cell Lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of CNOT4 in A549 lysate with CNOT4 antibody at (A) 1 and (B) 2 ug/ml.)

Related Product Information for anti-CNOT4 antibody

CNOT4 is a component of the CCR4-NOT transcription complex, a complex that is implicated in the repression of RNA polymerase II transcription. In the CCR4-NOT complex, CNOT4 acts as an E3 ubiquitin-protein ligase and interacts with a subset of E2 ubiquitin-conjugating enzymes through a unique C4C4 RING domain. This E3 ligase activity was shown to be dependent on the selective and specific interaction with the ubiquitin conjugating enzyme UbcH5B. In yeast, mutations in CNOT4 that prevented its interaction with the UbcH5B homolog UBC4 caused increased sensitivity to hydroxyurea, heat shock, and hygromycin B, suggesting that CNOT4 and UbcH5B are involved in stress response in vivo. Multiple isoforms of CNOT4 are known to exist.

Product Categories/Family for anti-CNOT4 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 300069013
• NCBI GeneID: 4850
• NCBI Accession #: NP_001177777.1
• NCBI GenBank Nucleotide #: NM_001190848.1
• UniProt Accession #: O95628; O95339; O95627; Q8IYM7; Q8NCL0; Q9NPQ1; Q9NZN6
• Molecular Weight: 63,510 Da
• NCBI Official Full Name: CCR4-NOT transcription complex subunit 4 isoform d
• NCBI Official Synonym Full Names: CCR4-NOT transcription complex, subunit 4
• NCBI Official Symbol: CNOT4
• NCBI Official Synonym Symbols: NOT4; NOT4H; CLONE243
• NCBI Protein Information: CCR4-NOT transcription complex subunit 4; OTTHUMP00000208245; OTTHUMP00000208246; OTTHUMP00000208247; OTTHUMP00000208248; OTTHUMP00000208540; CCR4-associated factor 4; E3 ubiquitin-protein ligase CNOT4; potential transcriptional repressor NOT4Hp; NOT4 (negative regulator of transcription 4, yeast) homolog
• UniProt Protein Name: CCR4-NOT transcription complex subunit 4
• Protein Family: CCR4-NOT transcription complex
• UniProt Gene Name: CNOT4
• UniProt Synonym Gene Names: NOT4
• UniProt Entry Name: CNOT4_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of the CCR4-NOT complex, a global transcriptional regulator. The encoded protein interacts with CNOT1 and has E3 ubiquitin ligase activity. Several transcript variants encoding different isoforms have been found for this gene.

Uniprot Description

CNOT4: Has E3 ubiquitin ligase activity. The CCR4-NOT complex functions as general transcription regulation complex. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; EC 6.3.2.19; EC 6.3.2.-; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: cytosol; nucleus; CCR4-NOT complex

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; nucleotide binding; ligase activity

Biological Process: poly(A) tail shortening; protein autoubiquitination; gene expression; mRNA catabolic process, deadenylation-dependent decay