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Western Blot (WB) (Western blot analysis of CNOT4 (arrow) using rabbit polyclonal CNOT4 Antibody (N-term) (RB11916). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CNOT4 gene (Lane 2) (Origene Technologies).)

Rabbit CNOT4 Polyclonal Antibody | anti-CNOT4 antibody

CNOT4 Antibody (N-term)

Gene Names
CNOT4; NOT4; NOT4H; CLONE243
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CNOT4; Polyclonal Antibody; CNOT4 Antibody (N-term); CCR4-NOT transcription complex subunit 4; 632-; CCR4-associated factor 4; E3 ubiquitin-protein ligase CNOT4; Potential transcriptional repressor NOT4Hp; NOT4; anti-CNOT4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CNOT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-84 amino acids from the N-terminal region of human CNOT4.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
54-84
Sequence Length
713
Applicable Applications for anti-CNOT4 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of CNOT4 (arrow) using rabbit polyclonal CNOT4 Antibody (N-term) (RB11916). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CNOT4 gene (Lane 2) (Origene Technologies).)

Western Blot (WB) (Western blot analysis of CNOT4 (arrow) using rabbit polyclonal CNOT4 Antibody (N-term) (RB11916). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CNOT4 gene (Lane 2) (Origene Technologies).)

Western Blot (WB)

(CNOT4 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CNOT4 antibody detected the CNOT4 protein (arrow).)

Western Blot (WB) (CNOT4 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CNOT4 antibody detected the CNOT4 protein (arrow).)
Related Product Information for anti-CNOT4 antibody
CNOT4 has intrinsic E3 ubiquitin ligase activity. Binding of the CNOT4 RING finger to the ubiquitin-conjugating enzyme (E2) UbcH5B is highly selective.
The CCR4-NOT complex functions as a global regulator of RNA polymerase II transcription.
Product Categories/Family for anti-CNOT4 antibody
References
Albert T.K., Nucleic Acids Res. 28:809-817(2000).
Albert T.K., EMBO J. 21:355-364(2002).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
63510
NCBI Official Full Name
CCR4-NOT transcription complex subunit 4 isoform b
NCBI Official Synonym Full Names
CCR4-NOT transcription complex, subunit 4
NCBI Official Symbol
CNOT4
NCBI Official Synonym Symbols
NOT4; NOT4H; CLONE243
NCBI Protein Information
CCR4-NOT transcription complex subunit 4
UniProt Protein Name
CCR4-NOT transcription complex subunit 4
UniProt Gene Name
CNOT4
UniProt Synonym Gene Names
NOT4
UniProt Entry Name
CNOT4_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of the CCR4-NOT complex, a global transcriptional regulator. The encoded protein interacts with CNOT1 and has E3 ubiquitin ligase activity. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010]

Uniprot Description

CNOT4: Has E3 ubiquitin ligase activity. The CCR4-NOT complex functions as general transcription regulation complex. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.-; Ligase

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: cytosol; nucleus; CCR4-NOT complex

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; nucleotide binding; ligase activity

Biological Process: poly(A) tail shortening; protein autoubiquitination; gene expression; mRNA catabolic process, deadenylation-dependent decay

Research Articles on CNOT4

Similar Products

Product Notes

The CNOT4 cnot4 (Catalog #AAA9208669) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 54-84. The CNOT4 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's CNOT4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the CNOT4 cnot4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CNOT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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