Rabbit anti-Human CNOT4 Polyclonal Antibody | anti-CNOT4 antibody

CNOT4, NT (E3 Ubiquitin-protein Ligase CNOT4, CCR4-associated Factor 4, Potential Transcriptional Repressor NOT4Hp, CCR4-NOT Transcription Complex Subunit 4, NOT4)

Cat. Num. AAA6009563

• Gene Names: CNOT4; NOT4; NOT4H; CLONE243
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: CNOT4; Polyclonal Antibody; NT (E3 Ubiquitin-protein Ligase CNOT4; CCR4-associated Factor 4; Potential Transcriptional Repressor NOT4Hp; CCR4-NOT Transcription Complex Subunit 4; NOT4); Anti -CNOT4; anti-CNOT4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human CNOT4. Species sequence homology: Mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-CNOT4 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1-2ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
• Immunogen: Synthetic peptide corresponding to 19aa near the N-terminus of the human CNOT4.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot analysis of CNOT4 in A549 lysate with CNOT4 antibody at (A) 1 and (B) 2ug/ml.)

Related Product Information for anti-CNOT4 antibody

CNOT4 is a 575aa protein with E3 ubiquitin ligase activity, mainly involved in protein modification and protein ubiquitination. It contains a ring type zinc finger domain of the C4C4 type, an RNA recognition motif and a bipartite nuclear localization signal. Studies show that CNOT4 is a component of the CCR4-NOT (CAF1) core complex that functions as general transcription regulation complex and has both a positive as well as a negative effect on the transcription of multiple functionally unrelated genes, thus controls gene expression. CNOT4 interacts with CNOT1 via C-terminus and with E2 ubiquitin ligases via C4C4 RING domain. Reports suggest that CNOT4 gets phosphorylated in response to DNA damage on consensus sites recognized by ATM and ATR.

Product Categories/Family for anti-CNOT4 antibody

Antibodies; Abs to Ubiquitin Ligases

NCBI and Uniprot Product Information

• NCBI GI #: 300069017
• NCBI GeneID: 4850
• NCBI Accession #: NP_001177779.1
• NCBI GenBank Nucleotide #: NM_001190850.1
• UniProt Accession #: O95628; O95339; O95627; Q8IYM7; Q8NCL0; Q9NPQ1; Q9NZN6; B7Z6I4; E7ET38; F8VQP3
• Molecular Weight: 63,510 Da
• NCBI Official Full Name: CCR4-NOT transcription complex subunit 4 isoform f
• NCBI Official Synonym Full Names: CCR4-NOT transcription complex, subunit 4
• NCBI Official Symbol: CNOT4
• NCBI Official Synonym Symbols: NOT4; NOT4H; CLONE243
• NCBI Protein Information: CCR4-NOT transcription complex subunit 4; CCR4-associated factor 4; E3 ubiquitin-protein ligase CNOT4; potential transcriptional repressor NOT4Hp; NOT4 (negative regulator of transcription 4, yeast) homolog
• UniProt Protein Name: CCR4-NOT transcription complex subunit 4
• Protein Family: CCR4-NOT transcription complex
• UniProt Gene Name: CNOT4
• UniProt Synonym Gene Names: NOT4
• UniProt Entry Name: CNOT4_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of the CCR4-NOT complex, a global transcriptional regulator. The encoded protein interacts with CNOT1 and has E3 ubiquitin ligase activity. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010]

Uniprot Description

CNOT4: Has E3 ubiquitin ligase activity. The CCR4-NOT complex functions as general transcription regulation complex. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.19; Ubiquitin conjugating system; Ligase; EC 6.3.2.-; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: cytosol; nucleus; CCR4-NOT complex

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; nucleotide binding; ligase activity

Biological Process: poly(A) tail shortening; protein autoubiquitination; gene expression; mRNA catabolic process, deadenylation-dependent decay