Western Blot (WB)
Rabbit anti-Human CCNB1 Polyclonal Antibody | anti-CCNB1 antibody
Anti-CCNB1 Antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purification
Synonyms
CCNB1; Polyclonal Antibody; Anti-CCNB1 Antibody; anti-CCNB1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Western blot analysis of extracts of various cell lines, using CCNB1 antibody. Immunofluorescence analysis of A549 cell using CCNB1 antibody. Blue: DAPI for nuclear staining.
Purity/Purification
Affinity Purification
Form/Format
Liquid
Concentration
1mg/ml (varies by lot)
Sequence Length
175
Applicable Applications for anti-CCNB1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50- 1:200
ICC: 1:50- 1:200
IF: 1:50- 1:200
IP: 1:20- 1:100
IHC: 1:50- 1:200
ICC: 1:50- 1:200
IF: 1:50- 1:200
IP: 1:20- 1:100
Immunogen
A synthetic peptide of human CCNB1
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.
Western Blot (WB)
Western Blot (WB)
Immunofluorescence (IF)
Immunofluorescence (IF)
Related Product Information for anti-CCNB1 antibody
Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) (1, 2) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C (3-5). Four cyclin B1 phosphorylation sites (Ser126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis (6-9). While MPF itself can phosphorylate Ser126 and Ser128, polo-like kinase 1 (PLK1) phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 (6, 10). At the end of mitosis, cyclin B1 is targeted for degradation by the anaphase-promoting complex (APC), allowing for cell cycle progression (11). Research studies have shown that cyclin B1 is overexpressed in breast, prostate, and non-small cell lung cancers (12-14).
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
19,026 Da
NCBI Official Full Name
CCNB1, partial
UniProt Protein Name
CCNB1
Protein Family
UniProt Gene Name
CCNB1
Similar Products
Product Notes
The CCNB1 ccnb1 (Catalog #AAA419959) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CCNB1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CCNB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500 - 1:2000 IHC: 1:50- 1:200 ICC: 1:50- 1:200 IF: 1:50- 1:200 IP: 1:20- 1:100. Researchers should empirically determine the suitability of the CCNB1 ccnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCNB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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