Rabbit anti-Human CB2 Polyclonal Antibody | anti-CB2 antibody

CB2, CT (CNR2, Cannabinoid receptor 2, CX5) (Azide free) (HRP)

Cat. Num. AAA6281083

• Gene Names: CNR2; CB2; CX5; CB-2
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CB2; Polyclonal Antibody; CT (CNR2; Cannabinoid receptor 2; CX5) (Azide free) (HRP); CNR2; CX5; anti-CB2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CB2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CB2 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 329-356 in the C-terminal region of human CB2, UniProt Accession #P34972.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS643300 in A431 cell line lysates (35ug/lane). This demonstrates the CB2 antibody detected the CB2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of MBS643300 in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MBS643300 using Jurkat cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CB2 antibody

The cannabinoid delta-9-tetrahydrocannabinol is the principal psychoactive ingredient of marijuana. The proteins encoded by this gene and the cannabinoid receptor 1 (brain) (CNR1) gene have the characteristics of a guanine nucleotide-binding protein (G-protein)-coupled receptor for cannabinoids. They inhibit adenylate cyclase activity in a dose-dependent, stereoselective, and pertussis toxin-sensitive manner. These proteins have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. The cannabinoid receptors are members of family 1 of the G-protein-coupled receptors.

Product Categories/Family for anti-CB2 antibody

Antibodies; Receptors

References

1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 2. Larrinaga, G., et al. Histol. Histopathol. 25(9):1133-1138(2010). 3. Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010). 4. Taylor, A.H., et al. Histochem. Cell Biol. 133(5):557-565(2010). 5. De Jesus, M.L., et al. Neurochem. Int. 56 (6-7), 829-833 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4502929
• NCBI GeneID: 1269
• NCBI Accession #: NP_001832.1
• NCBI GenBank Nucleotide #: NM_001841.2
• UniProt Accession #: P34972; Q4VBK8; Q5JRH7; Q6B0G7; Q6NSY0; C6ES44
• Molecular Weight: 39,681 Da
• NCBI Official Full Name: cannabinoid receptor 2
• NCBI Official Synonym Full Names: cannabinoid receptor 2 (macrophage)
• NCBI Official Symbol: CNR2
• NCBI Official Synonym Symbols: CB2; CX5; CB-2
• NCBI Protein Information: cannabinoid receptor 2; testis-dominant CNR2 isoform CB2
• UniProt Protein Name: Cannabinoid receptor 2
• UniProt Gene Name: CNR2
• UniProt Synonym Gene Names: CB2A; CB2B; CB-2; CB2; hCB2
• UniProt Entry Name: CNR2_HUMAN

NCBI Description

The cannabinoid delta-9-tetrahydrocannabinol is the principal psychoactive ingredient of marijuana. The proteins encoded by this gene and the cannabinoid receptor 1 (brain) (CNR1) gene have the characteristics of a guanine nucleotide-binding protein (G-protein)-coupled receptor for cannabinoids. They inhibit adenylate cyclase activity in a dose-dependent, stereoselective, and pertussis toxin-sensitive manner. These proteins have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. The cannabinoid receptors are members of family 1 of the G-protein-coupled receptors. [provided by RefSeq, Jul 2008]

Uniprot Description

CNR2: Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, family 1

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: extrinsic to internal side of plasma membrane; integral to plasma membrane; dendrite; plasma membrane; perikaryon

Molecular Function: cannabinoid receptor activity

Biological Process: negative regulation of mast cell activation; G-protein signaling, coupled to cyclic nucleotide second messenger; negative regulation of nitric-oxide synthase activity; negative regulation of inflammatory response; response to amphetamine; response to lipopolysaccharide; immune response; negative regulation of action potential; negative regulation of synaptic transmission, GABAergic; inflammatory response; sensory perception of pain