Western Blot (WB)
(All lanes : Anti-ADRA2B Antibody (Center) at 1:1000 dilutionLane 1: MCF-7 whole cell lysateLane 2: MDA-MB-231 whole cell lysateLane 3: SK-BR-3 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human ADRA2B Polyclonal Antibody | anti-ADRA2B antibody
ADRA2B Antibody (Center)
IHC-P~~1:50~100
FC~~1:10~50
IF~~1:10~50
Western Blot (WB)
(All lanes : Anti-ADRA2B Antibody (Center) at 1:1000 dilutionLane 1: MCF-7 whole cell lysateLane 2: MDA-MB-231 whole cell lysateLane 3: SK-BR-3 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-ADRA2B Antibody (Center) at 1:1000 dilutionLane 1: MCF-7 whole cell lysateLane 2: MDA-MB-231 whole cell lysateLane 3: SK-BR-3 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human breast carcinoma with ADRA2B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human breast carcinoma with ADRA2B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of MDA-231 cells using ADRA2B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of MDA-231 cells using ADRA2B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of ADRA2B Antibody (Center) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of ADRA2B Antibody (Center) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
NCBI and Uniprot Product Information
NCBI Description
This intronless gene encodes a seven-pass transmembrane protein. This protein is a member of a subfamily of G protein-coupled receptors that regulate neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. [provided by RefSeq, Apr 2014]
Uniprot Description
ADRA2B: Alpha-2 adrenergic receptors mediate the catecholamine- induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is clonidine > norepinephrine > epinephrine = oxymetazoline > dopamine > p-tyramine = phenylephrine > serotonin > p-synephrine / p-octopamine. For antagonists, the rank order is yohimbine > chlorpromazine > phentolamine > mianserine > spiperone > prazosin > alprenolol > propanolol > pindolol. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2B sub-subfamily.
Protein type: Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1; Membrane protein, integral
Chromosomal Location of Human Ortholog: 2q11.1
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: protein binding; alpha2-adrenergic receptor activity; epinephrine binding
Biological Process: G-protein coupled receptor protein signaling pathway; platelet activation; cell-cell signaling; activation of protein kinase B; regulation of vascular smooth muscle contraction; negative regulation of epinephrine secretion; MAPKKK cascade; female pregnancy; angiogenesis; blood coagulation; positive regulation of neuron differentiation; positive regulation of blood pressure
Disease: Epilepsy, Familial Adult Myoclonic, 2
Research Articles on ADRA2B
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Product Notes
The ADRA2B adra2b (Catalog #AAA9213197) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 343-369. The ADRA2B Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ADRA2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-paraffin (IHC-P), Flow Cytometry (FC/FACS), Immunofluorescence (IF). WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 IF~~1:10~50. Researchers should empirically determine the suitability of the ADRA2B adra2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADRA2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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