Rabbit anti-Human Catechol-O-Methyltransferase (COMT) Polyclonal Antibody | anti-COMT antibody

Polyclonal Antibody to Catechol-O-Methyltransferase (COMT)

Cat. Num. AAA2005715

• Gene Names: COMT; HEL-S-98n
• Reactivity: Human
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Catechol-O-Methyltransferase (COMT); Polyclonal Antibody; Polyclonal Antibody to Catechol-O-Methyltransferase (COMT); anti-COMT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against COMT. It has been selected for its ability to recognize COMT in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGSEF- GDTKEQRIL NHVLQHAEPG NAQSVLEAID TYCEQKEWAM NVGDKKGKIV DAVIQEHQPS VLLELGAYCG YSAVRMARLL SPGARLITIE INPDCAAITQ RMVDFAGVKD KVTLVVGASQ DIIPQLKKKY DVDTLDMVFL DHWKDRYLPD TLLLEECGLL RKGTVLLADN VICPGAPDFL AHVRGSSCFE CTHYQSFLEY REVVDGLEKA IYKGPGSEAG P
• Sequence Length: 27

• Applicable Applications for anti-COMT antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant COMT (Gly52~Pro271) expressed in E.coli.
• Cross Reactivity: Human
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2081272
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Human A549 Cells; Lane2: Human MCF7 Cells; Lane3: Human Liver Tissue; Lane4: Human 293T Cells.)

Western Blot (WB)

(Western Blot: Sample: Recombinant COMT, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P. Samples: Human Tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 4502969
• NCBI GeneID: 1312
• NCBI Accession #: NP_000745.1
• NCBI GenBank Nucleotide #: NM_000754.3
• UniProt Accession #: P21964; Q6IB07; Q6ICE6; Q9BWC7; A8MPV9
• Molecular Weight: 24,449 Da
• NCBI Official Full Name: catechol O-methyltransferase isoform MB-COMT
• NCBI Official Synonym Full Names: catechol-O-methyltransferase
• NCBI Official Symbol: COMT
• NCBI Official Synonym Symbols: HEL-S-98n
• NCBI Protein Information: catechol O-methyltransferase
• UniProt Protein Name: Catechol O-methyltransferase
• Protein Family: Catechol O-methyltransferase
• UniProt Gene Name: COMT

NCBI Description

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008]

Uniprot Description

COMT: Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. Belongs to the mammalian catechol-O-methyltransferase family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Amino Acid Metabolism - tyrosine; EC 2.1.1.6; Membrane protein, integral; Methyltransferase

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: axon; cell body; cytosol; dendritic spine; extracellular exosome; integral component of membrane; membrane; mitochondrion; plasma membrane; postsynaptic membrane

Molecular Function: catechol O-methyltransferase activity; L-dopa O-methyltransferase activity; magnesium ion binding; methyltransferase activity; O-methyltransferase activity; orcinol O-methyltransferase activity; protein binding

Biological Process: cellular response to phosphate starvation; developmental process; dopamine catabolic process; estrogen metabolic process; female pregnancy; learning; methylation; multicellular organismal reproductive process; negative regulation of dopamine metabolic process; negative regulation of smooth muscle cell proliferation; neurotransmitter catabolic process; positive regulation of homocysteine metabolic process; regulation of sensory perception of pain; response to drug; response to lipopolysaccharide; response to organic cyclic compound; response to pain; short-term memory

Disease: Panic Disorder 1; Schizophrenia