Human COMT ELISA Kit | COMT elisa kit

Human COMT ELISA Kit

Cat. Num. AAA8248346

• Gene Names: COMT; HEL-S-98n
• Reactivity: Human
• Synonyms: COMT; Human COMT ELISA Kit; Catechol O-methyltransferase; COMT elisa kit

• Reactivity: Human
• Specificity: The Human COMT ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human COMT proteins within the range of 31.2 pg/ml - 2000 pg/ml.
• Sequence Length: 271

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COMT elisa kit

Principle of the Assay: The Human COMT ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human COMT in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human COMT coated on a 96-well plate. Standards and samples are pipetted into the wells and COMT present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human COMT antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of COMT bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Catechol-O-methyltransferase (COMT) is one of several enzymes that degrade catecholamines such as dopamine, epinephrine, and norepinephrine. In humans, catechol-O-methyltransferase protein is encoded by the COMT gene. As the regulation of catecholamines is impaired in a number of medical conditions, several pharmaceutical drugs target COMT to alter its activity and therefore the availability of catecholamines. In the brain, COMT-dependent dopamine degradation is of particular importance in brain regions with low expression of the presynaptic dopamine transporter (DAT), such as the prefrontal cortex. This process is supposed to take place in postsynaptic neurons, as, in general, COMT is located intracellularly in the central nervous system (CNS).

NCBI and Uniprot Product Information

• NCBI GI #: 4502969
• NCBI GeneID: 1312
• NCBI Accession #: NP_000745.1
• NCBI GenBank Nucleotide #: NM_000754.3
• UniProt Accession #: P21964; Q6IB07; Q6ICE6; Q9BWC7; A8MPV9
• Molecular Weight: 24,449 Da
• NCBI Official Full Name: catechol O-methyltransferase isoform MB-COMT
• NCBI Official Synonym Full Names: catechol-O-methyltransferase
• NCBI Official Symbol: COMT
• NCBI Official Synonym Symbols: HEL-S-98n
• NCBI Protein Information: catechol O-methyltransferase
• UniProt Protein Name: Catechol O-methyltransferase
• Protein Family: Catechol O-methyltransferase
• UniProt Gene Name: COMT
• UniProt Entry Name: COMT_HUMAN

NCBI Description

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008]

Uniprot Description

COMT: Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. Belongs to the mammalian catechol-O-methyltransferase family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Amino Acid Metabolism - tyrosine; EC 2.1.1.6; Membrane protein, integral; Methyltransferase

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: cytosol; membrane; plasma membrane

Molecular Function: catechol O-methyltransferase activity; methyltransferase activity; O-methyltransferase activity; protein binding

Biological Process: methylation

Disease: Panic Disorder 1; Schizophrenia