Human COMT PicoKine ELISA Kit | COMT elisa kit

Human COMT PicoKine ELISA Kit

Cat. Num. AAA177607

• Gene Names: COMT; HEL-S-98n
• Reactivity: Human
• Synonyms: COMT PicoKine; Human COMT PicoKine ELISA Kit; Catechol O-methyltransferase; Catechol O methyltransferase; COMT; COMT_HUMAN; EC 2.1.1.6 ; catechol-O-methyltransferase; COMT elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human COMT
• Sequence Length: 271

• Assay Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of human COMT in cell culture supernates, serum and plasma(heparin, EDTA).
• Immunogen: E Coli, G52-P271
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human COMT standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum and plasma (heparin, EDTA) to each empty well. It is recommended that each human COMT standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|305|818|1249|254|699|1358; Standard deviation|15.55|43.35|77.43|16.25|38.44|92.34; CV(%)|5.1|5.3|6.2|6.4|5.5|6.8
• Sample Data: Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000; O.D.|0.036|0.085|0.143|0.248|0.444|0.750|1.234|2.101
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human COMT PicoKine ELISA Kit standard curve )

Related Product Information for COMT elisa kit

Principle of the assay: Human COMT ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for COMT has been precoated onto 96-well plates. Standards (E Coli, G52-P271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for COMT is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human COMT amount of sample captured in plate.

Background: Catechol-O-methyltransferase (COMT) is one of several enzymes that degrade catecholamines such as dopamine, epinephrine, and norepinephrine. In humans, catechol-O-methyltransferase protein is encoded by the COMT gene. As the regulation of catecholamines is impaired in a number of medical conditions, several pharmaceutical drugs target COMT to alter its activity and therefore the availability of catecholamines. In the brain, COMT-dependent dopamine degradation is of particular importance in brain regions with low expression of the presynaptic dopamine transporter (DAT), such as the prefrontal cortex. This process is supposed to take place in postsynaptic neurons, as, in general, COMT is located intracellularly in the central nervous system (CNS).

NCBI and Uniprot Product Information

• NCBI GI #: 4502969
• NCBI GeneID: 1312
• NCBI Accession #: NP_000745.1
• NCBI GenBank Nucleotide #: NM_000754.3
• UniProt Accession #: P21964; Q6IB07; Q6ICE6; Q9BWC7; A8MPV9
• Molecular Weight: 24,449 Da
• NCBI Official Full Name: catechol O-methyltransferase isoform MB-COMT
• NCBI Official Synonym Full Names: catechol-O-methyltransferase
• NCBI Official Symbol: COMT
• NCBI Official Synonym Symbols: HEL-S-98n
• NCBI Protein Information: catechol O-methyltransferase
• UniProt Protein Name: Catechol O-methyltransferase
• Protein Family: Catechol O-methyltransferase
• UniProt Gene Name: COMT
• UniProt Entry Name: COMT_HUMAN

NCBI Description

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008]

Uniprot Description

COMT: Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. Belongs to the mammalian catechol-O-methyltransferase family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: EC 2.1.1.6; Methyltransferase; Amino Acid Metabolism - tyrosine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: axon; cytosol; dendritic spine; integral to membrane; membrane; mitochondrion; plasma membrane; postsynaptic membrane

Molecular Function: catechol O-methyltransferase activity; magnesium ion binding; methyltransferase activity; O-methyltransferase activity; protein binding

Biological Process: cellular response to phosphate starvation; developmental process; dopamine catabolic process; estrogen metabolic process; female pregnancy; learning; methylation; negative regulation of dopamine metabolic process; negative regulation of smooth muscle cell proliferation; neurotransmitter catabolic process; positive regulation of homocysteine metabolic process; regulation of sensory perception of pain; reproductive process in a multicellular organism; response to drug; response to lipopolysaccharide; response to organic cyclic substance; response to pain; short-term memory

Disease: Panic Disorder 1; Schizophrenia