Rabbit Caspase 6, cleaved, active Polyclonal Antibody | anti-Casp6 antibody

Caspase 6, cleaved, active (Mch2)

Cat. Num. AAA612416

• Gene Names: Casp6; Mch2; MGC93335
• Reactivity: Canine, Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Serum; Serum
• Synonyms: Caspase 6; cleaved; active; Polyclonal Antibody; active (Mch2); Anti -Caspase 6; anti-Casp6 antibody

• Host: Rabbit
• Reactivity: Canine, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: 1. Caspase antibodies are classical tools for detecting inactive (pro) and active (cleaved) forms of the enzymes. The presence of the large or small subunits in western blots ia considered to be a marker of caspase activation. IMG-5701 preferentially detects active caspase-6 (large subunit and small subunits). However, it may also detect pro-caspase-6 or other intermediate cleavage fragments in some cell or tissue systems. Species Crossreactivity: Cross reacts with canine, Gerbil, Human, Mouse and Rat.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

• Applicable Applications for anti-Casp6 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry (Fr) (paraffin-embedded sections).; Dilution: Western Blot: 1:1000-1:2000.; Immunohistochemistry (paraffin-embedded sections): 1:1000-1:5000.; Immunoprecipitation: 1:50-1:200.
• Immunogen: Recombinant catalytically active human Caspase-6 protein was used as immunogen.
• Positive Control: Cells or tissues undergoing apoptosis, CNS, liver, spleen.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Casp6 antibody

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are constitutively expressed in almost all cell types as inactive proenzymes (zymogens: enzyme precursors which require a biochemical change to become active enzymes) that are processed and activated in response to a variety of pro-apoptotic or inflammatory stimuli. The procaspases (32-56kD) contain four domains: an N-terminal prodomain (2-25kD), a large subunit (p20: 17-21kD), a small subunit (p10: 10-13kD) and a short linker region between the large and small subunits. Caspase activation involves proteolytic processing of the proenzyme at specific aspartate residues between the domains. This results in removal of the prodomain as well as the linker region and formation of a heterodimer containing one large and one small subunit (p20-p10). The active caspase is a tetramer composed of two heterodimers (p202-p102). Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. The Active/Cleaved Caspase-6 IMG-5701 polyclonal antisera recognizes the large and small subunits of active/cleaved caspase-6. Whereas the antisera has a strong preference for active/cleaved caspase-6, in some cell or tissue systems or techniques the antisera may also recognize the ~34kD proform of caspase-6 as well as intermediate caspase-6 cleavage fragments.

Product Categories/Family for anti-Casp6 antibody

Antibodies; Abs to Apoptosis; Caspase

NCBI and Uniprot Product Information

• NCBI GI #: 51491891
• NCBI GeneID: 83584
• NCBI Accession #: NP_113963.2
• NCBI GenBank Nucleotide #: NM_031775.2
• UniProt Accession #: O35397; Q6AZ23
• Molecular Weight: 31,556 Da
• NCBI Official Full Name: caspase 6
• NCBI Official Synonym Full Names: caspase 6
• NCBI Official Symbol: Casp6
• NCBI Official Synonym Symbols: Mch2; MGC93335
• NCBI Protein Information: caspase-6; CASP-6; apoptotic protease Mch-2
• UniProt Protein Name: Caspase-6
• Protein Family: Caspase
• UniProt Gene Name: Casp6
• UniProt Synonym Gene Names: Mch2
• UniProt Entry Name: CASP6_RAT

NCBI Description

member of the cysteine-aspartic acid protease (caspase) family that plays a terminal role in apoptosis [RGD]

Uniprot Description

CASP6: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce. Activation is suppressed by phosphorylation at Ser-257. Belongs to the peptidase C14A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Apoptosis; EC 3.4.22.59

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: identical protein binding; cysteine-type endopeptidase activity; endopeptidase activity

Biological Process: lens development in camera-type eye; response to metal ion; positive regulation of apoptosis; positive regulation of retinal cell programmed cell death; apoptosis; acute inflammatory response to non-antigenic stimulus; response to testosterone stimulus; response to organic nitrogen; proteolysis; response to estradiol stimulus; response to organic substance; response to hydrogen peroxide; epithelial cell differentiation; response to heat; positive regulation of neuron apoptosis; response to cytokine stimulus; response to glucose stimulus; response to gamma radiation; response to iron ion