Rabbit Caspase-3 Polyclonal Antibody | anti-Casp3 antibody

Polyclonal Antibody to Caspase-3 (active/cleaved)

Cat. Num. AAA668572

• Gene Names: Casp3; CC3; AC-3; Lice; Yama; mldy; CPP32; SCA-1; CASP-3; CPP-32; Caspase-3; A830040C14Rik
• Reactivity: Dog, Gerbil, Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Serum
• Synonyms: Caspase-3; Polyclonal Antibody; Polyclonal Antibody to Caspase-3 (active/cleaved); Casp3; Cpp32; Apopain; CPP-32; Cysteine protease CPP32; LICE; Protein Yama; SCA-1; SREBP cleavage activity 1; Antibody to Caspase-3 (active/cleaved); Anti-Caspase-3 (active/cleaved) Antibody; Caspase-3 (active/cleaved) Antibody; anti-Casp3 antibody

• Host: Rabbit
• Reactivity: Dog, Gerbil, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Serum
• Form/Format: 50 ul sera
• Sequence Length: 277

• Applicable Applications for anti-Casp3 antibody: Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP)
• Application Notes: WB: 1: 1000-1: 2000; IHC (paraffin): 1: 1000-1: 5000; IP: 1: 50-1: 200
• Immunogen Information: A recombinant catalytically active human caspase-3 protein was used as immunogen for this antibody
• Antigen: Caspase-3 (active/cleaved)
• Preparation and Storage: Store the antibody at 4 degree C, stable for 6 months.; For long-term storage, store at -20 degree C.; Avoid repeated freeze and thaw cycles.

Immunohistochemistry (IHC)

(Fig: 1 Formalin-fixed, paraffin-embedded section of dog ischemic brain stained for Active/Cleaved Caspase-3 expression using at 1: 2000. Staining is seen in the nuclei of dying neurons (black arrow) but not in the morphologically normal nuclei (open arrows). Caspase-3 expression in the nucleus is considered to be a marker of active/caspase-3 expression and apoptosis. Hematoxylin-eosin counterstain.)

Immunohistochemistry (IHC)

(Fig: 2 Formalin-fixed, paraffin-embedded section of irradiated mouse spleen stained for Active/Cleaved Caspase-3 expression using at 1: 2000. Staining is seen in the nuclei of a subset of the cell population. Caspase-3 expression in the nucleus is considered to be a marker of active/caspase-3 expression and apoptosis. Hematoxylin-eosin counterstain.)

Related Product Information for anti-Casp3 antibody

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases that consist of the heterotetramer p202-p102. Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. The Caspase-3 polyclonal antisera recognizes the proform of caspase-3 (approx. 32) kDa), and the large (approx. 14-21 kDa) and small (approx. 10 kDa) subunits of active/cleaved Caspase-3.

Product Categories/Family for anti-Casp3 antibody

Antibodies; Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 2493527
• NCBI GeneID: 12367
• UniProt Accession #: P70677; O08668; Q8CHV5; Q9QWI4
• Molecular Weight: 31,475 Da
• NCBI Official Full Name: Caspase-3
• NCBI Official Synonym Full Names: caspase 3
• NCBI Official Symbol: Casp3
• NCBI Official Synonym Symbols: CC3; AC-3; Lice; Yama; mldy; CPP32; SCA-1; CASP-3; CPP-32; Caspase-3; A830040C14Rik
• NCBI Protein Information: caspase-3
• UniProt Protein Name: Caspase-3
• Protein Family: Caspase
• UniProt Gene Name: Casp3
• UniProt Synonym Gene Names: Cpp32; CASP-3; CPP-32; SCA-1

NCBI Description

This gene encodes a protein that belongs to a highly conserved family of cysteinyl aspartate-specific proteases that function as essential regulators of programmed cell death through apoptosis. Members of this family contain an N-terminal pro-domain and require cleavage at specific aspartate residues to become mature. The protein encoded by this gene belongs to a subgroup of cysteinyl aspartate-specific proteases that are activated by initiator caspases and that perform the proteolytic cleavage of apoptotic target proteins. Mice defective for this gene exhibit a variety of phenotypes including reduced neuronal apoptosis resulting in hyperplasias, hearing loss, attenuated osteogenic differentiation of bone marrow stromal stem cells, and pre- and post-natal lethality. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family.

Protein type: Apoptosis; EC 3.4.22.56; Motility/polarity/chemotaxis; Protease

Chromosomal Location of Human Ortholog: 8 B1.1|8 26.39 cM

Cellular Component: cytoplasm; cytosol; lipid raft; nucleus

Molecular Function: aspartic-type endopeptidase activity; cyclin-dependent protein kinase inhibitor activity; cysteine-type endopeptidase activity; cysteine-type peptidase activity; death receptor binding; peptidase activity; phospholipase A2 activator activity; protease binding; protein complex binding

Biological Process: apoptosis; B cell homeostasis; cell fate commitment; erythrocyte differentiation; heart development; keratinocyte differentiation; learning and/or memory; negative regulation of activated T cell proliferation; negative regulation of apoptosis; negative regulation of B cell proliferation; negative regulation of cell cycle; negative regulation of cyclin-dependent protein kinase activity; nerve growth factor receptor signaling pathway; neuron apoptosis; neuron differentiation; positive regulation of apoptosis; positive regulation of neuron apoptosis; protein processing; proteolysis; response to DNA damage stimulus; response to glucose stimulus; response to hydrogen peroxide; response to organic substance; response to UV; response to wounding; sensory perception of sound; T cell homeostasis