Rabbit anti-Human SYN3 Polyclonal Antibody | anti-SYN3 antibody

SYN3, ID (SYN3, Synapsin-3, Synapsin III) (Biotin)

Cat. Num. AAA6352854

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SYN3; Polyclonal Antibody; ID (SYN3; Synapsin-3; Synapsin III) (Biotin); Synapsin III; anti-SYN3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-SYN3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SYN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 370-399 amino acids from the Central region of human SYN3.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SYN3 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (15ug/lane).This demonstrates the SYN3 antibody detected the SYN3 protein (arrow).)

Immunohistochemistry (IHC)

(SYN3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SYN3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-SYN3 antibody

This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. The protein encoded by this gene shares the synapsin family domain model, with domains A, C, and E exhibiting the highest degree of conservation. The protein contains a unique domain J, located between domains C and E. Based on this gene's localization to 22q12.3, a possible schizophrenia susceptibility locus, and the established neurobiological roles of the synapsins, this family member may represent a candidate gene for schizophrenia. The TIMP3 gene is located within an intron of this gene and is transcribed in the opposite direction.

Product Categories/Family for anti-SYN3 antibody

Antibodies; Neuroscience

References

Tsai, M.T., et al. Am. J. Med. Genet. 114(1):79-83(2002). Kao, H.T., et al. J. Exp. Zool. 285(4):360-377(1999). Porton, B., et al. J. Neurochem. 73(6):2266-2271(1999). Kao, H.T., et al. Proc. Natl. Acad. Sci. U.S.A. 95(8):4667-4672(1998).

NCBI and Uniprot Product Information

• NCBI GI #: 209413752
• NCBI GeneID: 8224
• NCBI Accession #: NP_003481.3
• NCBI GenBank Nucleotide #: NM_003490.3
• UniProt Accession #: O14994; B1B1F9
• Molecular Weight: 63,303 Da
• NCBI Official Full Name: synapsin-3 isoform IIIa
• UniProt Protein Name: Synapsin-3
• Protein Family: Synapsin
• UniProt Gene Name: SYN3
• UniProt Entry Name: SYN3_HUMAN

Uniprot Description

SYN3: neuronal phosphoprotein that may be involved in the regulation of neurotransmitter release and synaptogenesis. Binds ATP with high affinity and ADP with a lower affinity. This is consistent with a catalytic role of the C-domain in which ADP would be dissociated by cellular ATP after bound ATP was hydrolyzed. May represent a candidate gene for schizophrenia. Alternative splicing results in six variants; however, only three isoforms have been fully described.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: synaptic vesicle; synaptic vesicle membrane; cell junction

Molecular Function: catalytic activity; ATP binding

Biological Process: metabolic process; neurotransmitter secretion; regulation of synaptic transmission, GABAergic