Rabbit Caspase 3, Pro, Active/Cleaved Polyclonal Antibody | anti-CASP5 antibody

Caspase 3, Pro, Active/Cleaved (Caspase-3, CASP 3, CASP3, CASP-3, CPP-32, Apopain, Yama, SCA-1, ASP3, Apoptosis-related Cysteine Peptidase Apopain, Apopain, Cysteine Protease CPP32, SREBP Cleavage Activity 1, Yama, Yama Protein)

Cat. Num. AAA646981

• Gene Names: CASP5; ICH-3; ICEREL-III; ICE(rel)III
• Reactivity: Canine, Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Serum; Serum
• Synonyms: Caspase 3; Pro; Active/Cleaved; Polyclonal Antibody; Active/Cleaved (Caspase-3; CASP 3; CASP3; CASP-3; CPP-32; Apopain; Yama; SCA-1; ASP3; Apoptosis-related Cysteine Peptidase Apopain; Cysteine Protease CPP32; SREBP Cleavage Activity 1; Yama Protein); Anti -Caspase 3; anti-CASP5 antibody

• Host: Rabbit
• Reactivity: Canine, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the proform of human caspase-3 (~32kD), and the large (~14-21kD) and small (~10kD) subunits of active/cleaved Caspase-3. Species Crossreactivity: canine, gerbil, mouse, rat.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid, 0.05% sodium azide.

• Applicable Applications for anti-CASP5 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry (paraffin and frozen) and Immunoprecipitation.; Dilution: Western Blot: 1:1000-1:2000; Immunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000; Immunoprecipitation: 1:50-1:200; Immunohistochemistry: Frozen
• Immunogen: Recombinant corresponding to full-length human Caspase-3 protein (pro-form).
• Positive Control: Jurkat, most cancer cell lines, lymphoid tissue.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Caspase-3. Lysates from Jurkat cells (lane 1), normal mammary tissue (lane 2) and surgical specimens from three invasive ductal carcinomas (lanes 3-5) were normalized for total protein content (50ug/lane) and western blotted with anti-Caspase-3 . The ~32kD pro-Caspase-3 protein was detected in all samples. Active/cleaved Caspase-3 was identified in Jurkat (10kD small subunit, lane 1) and two ductal carcinomas (14-21kD large subunit).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of Caspase-3 expression in formalin-fixed, paraffin-embedded human reactive lymph node using at 1:2000. Staining is seen in the apoptosis-prone germinal center B lymphocytes of follicles. In constrast little or no staining is seen in the surrounding long-live mantle zone lymphocytes.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of Caspase-3 expression in formalin-fixed, paraffin-embedded human breast ductal carcinoma in situ using at 1:2000. Staining is seen in the the cancerous ducts, but not in the normal lobulus.)

Related Product Information for anti-CASP5 antibody

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases that consist of the heterotetramer p202-p102. Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes.

Product Categories/Family for anti-CASP5 antibody

Antibodies; Abs to Apoptosis; Caspase

NCBI and Uniprot Product Information

• NCBI GI #: 209870079
• NCBI GeneID: 838
• NCBI Accession #: NP_001129584.1
• NCBI GenBank Nucleotide #: NM_001136112.1
• UniProt Accession #: P51878; Q0QVY7; Q0QVY8; Q0QVZ0; Q0QVZ1; Q0QVZ2; Q14DD6; Q1HBJ3; Q6DJV7; B4DKP5
• Molecular Weight: 49,736 Da
• NCBI Official Full Name: caspase-5 isoform f
• NCBI Official Synonym Full Names: caspase 5, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP5
• NCBI Official Synonym Symbols: ICH-3; ICEREL-III; ICE(rel)III
• NCBI Protein Information: caspase-5; CASP-5; TY protease; protease TY; ICE(rel)-III; protease ICH-3; caspase 5, apoptosis-related cysteine protease
• UniProt Protein Name: Caspase-5
• Protein Family: Caspase
• UniProt Gene Name: CASP5
• UniProt Synonym Gene Names: ICH3; CASP-5
• UniProt Entry Name: CASP5_HUMAN

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein; this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

Function: Mediator of programmed cell death (apoptosis).

Catalytic activity: Strict requirement for Asp at the P1 position. It has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|- but also cleaves at Asp-Glu-Val-Asp-|-.

Subunit structure: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunits.

Tissue specificity: Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.

Induction: Up-regulated by bacterial lipopolysaccharides (LPS). Ref.1

Post-translational modification: The two subunits are derived from the precursor sequence by an autocatalytic mechanism.

Sequence similarities: Belongs to the peptidase C14A family.Contains 1 CARD domain.

Sequence caution: The sequence AAA75172.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAH74994.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAI13407.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence ABF47103.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAG59257.1 differs from that shown. Reason: Frameshift at position 10. The sequence CAA64450.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.