Human Microseminoprotein Beta (MSMb) ELISA Kit | MSMb elisa kit

Human Microseminoprotein Beta (MSMb) ELISA Kit

Cat. Num. AAA2020155

• Gene Names: MSMB; MSP; PSP; IGBF; MSPB; PN44; PRPS; HPC13; PSP57; PSP94; PSP-94
• Reactivity: Human
• Synonyms: Microseminoprotein Beta (MSMb); Human Microseminoprotein Beta (MSMb) ELISA Kit; MSM-B; IGBF; MSP; MSPB; PN44; PRPS; PSP; PSP57; PSP94; Immunoglobulin-binding factor; Prostate secreted seminal plasma protein; plasma beta-inhibin; MSMb elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Microseminoprotein Beta (MSMb).; No significant cross-reactivity or interference between Microseminoprotein Beta (MSMb) and analogues was observed.
• Sequence Length: 114

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Seminal Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.065ng/m
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Microseminoprotein Beta (MSMb) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Microseminoprotein Beta (MSMb) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MSMb elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microseminoprotein Beta (MSMb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microseminoprotein Beta (MSMb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microseminoprotein Beta (MSMb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Microseminoprotein Beta (MSMb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MSMb elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 119574070
• NCBI GeneID: 4477
• UniProt Accession #: P08118; P11999; Q13125; Q6IAY9; Q9UC59; B1API6
• Molecular Weight: 8,778 Da
• NCBI Official Full Name: microseminoprotein, beta-, isoform CRA_c
• NCBI Official Synonym Full Names: microseminoprotein, beta-
• NCBI Official Symbol: MSMB
• NCBI Official Synonym Symbols: MSP; PSP; IGBF; MSPB; PN44; PRPS; HPC13; PSP57; PSP94; PSP-94
• NCBI Protein Information: beta-microseminoprotein
• UniProt Protein Name: Beta-microseminoprotein
• Protein Family: Methanesulfonate monooxygenase hydroxylase
• UniProt Gene Name: MSMB
• UniProt Synonym Gene Names: PRSP; IGBF; PSP-94; PSP94
• UniProt Entry Name: MSMB_HUMAN

NCBI Description

The protein encoded by this gene is a member of the immunoglobulin binding factor family. It is synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. This protein has inhibin-like activity. It may have a role as an autocrine paracrine factor in uterine, breast and other female reproductive tissues. The expression of the encoded protein is found to be decreased in prostate cancer. Two alternatively spliced transcript variants encoding different isoforms are described for this gene. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MSMB: Defects in MSMB are the cause of susceptibility to prostate cancer hereditary type 13 (HPC13). It is a condition associated with familial predisposition to cancer of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma. Belongs to the beta-microseminoprotein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; nucleus

Disease: Prostate Cancer, Hereditary, 13