Western Blot (WB)
(Western Blot: Sample. Lane1: Mouse Spleen Tissue; Lane2: Mouse Liver Tissue; Lane3: Mouse Blood Cells.)
Rabbit anti-Mouse Caspase 3 (CASP3) Polyclonal Antibody | anti-CASP3 antibody
Polyclonal Antibody to Caspase 3 (CASP3)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SG IYLDSSYKMD YPEMGICIII NNKNFHKSTG MSSRSGTDVD AANLRETFMG LKYQVRNKND LTREDILELM DSVSKEDHSK RSSFVCVILS HGDEGVIYGT NGPVELKKLT SFFRGDYCRS LTGKPKLFII QACRGTELDC GIETD
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample. Lane1: Mouse Spleen Tissue; Lane2: Mouse Liver Tissue; Lane3: Mouse Blood Cells.)
Western Blot (WB)
(Western Blot: Sample. Lane1: Mouse Spleen Tissue; Lane2: Mouse Liver Tissue; Lane3: Mouse Blood Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CASP3, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CASP3, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that belongs to a highly conserved family of cysteinyl aspartate-specific proteases that function as essential regulators of programmed cell death through apoptosis. Members of this family contain an N-terminal pro-domain and require cleavage at specific aspartate residues to become mature. The protein encoded by this gene belongs to a subgroup of cysteinyl aspartate-specific proteases that are activated by initiator caspases and that perform the proteolytic cleavage of apoptotic target proteins. Mice defective for this gene exhibit a variety of phenotypes including reduced neuronal apoptosis resulting in hyperplasias, hearing loss, attenuated osteogenic differentiation of bone marrow stromal stem cells, and pre- and post-natal lethality. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Uniprot Description
CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family.
Protein type: Apoptosis; EC 3.4.22.56; Motility/polarity/chemotaxis; Protease
Chromosomal Location of Human Ortholog: 8 B1.1|8 26.39 cM
Cellular Component: cytoplasm; cytosol; membrane raft; nucleus
Molecular Function: aspartic-type endopeptidase activity; cyclin-dependent protein serine/threonine kinase inhibitor activity; cysteine-type endopeptidase activity; cysteine-type peptidase activity; death receptor binding; hydrolase activity; peptidase activity; phospholipase A2 activator activity; protease binding; protein binding; protein complex binding
Biological Process: apoptosis; B cell homeostasis; cell fate commitment; cellular response to DNA damage stimulus; cellular response to organic substance; erythrocyte differentiation; heart development; intrinsic apoptotic signaling pathway in response to osmotic stress; keratinocyte differentiation; learning and/or memory; negative regulation of activated T cell proliferation; negative regulation of apoptosis; negative regulation of B cell proliferation; negative regulation of cell cycle; negative regulation of cyclin-dependent protein serine/threonine kinase activity; nerve growth factor receptor signaling pathway; neuron apoptosis; neuron differentiation; positive regulation of apoptosis; positive regulation of neuron apoptotic process; protein processing; proteolysis; response to glucose stimulus; response to hydrogen peroxide; response to organic substance; response to UV; response to wounding; sensory perception of sound; T cell homeostasis
Research Articles on CASP3
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Product Notes
The CASP3 casp3 (Catalog #AAA2003999) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Caspase 3 (CASP3) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase 3 (CASP3) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CASP3 casp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SG IYLDSSYKMD YPEMGICIII NNKNFHKSTG MSSRSGTDVD AANLRETFMG LKYQVRNKND LTREDILELM DSVSKEDHSK RSSFVCVILS HGDEGVIYGT NGPVELKKLT SFFRGDYCRS LTGKPKLFII QACRGTELDC GIETD. It is sometimes possible for the material contained within the vial of "Caspase 3 (CASP3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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