Caspase 3 (CASP3) Recombinant Protein | CASP3 recombinant protein

Recombinant Caspase 3 (CASP3)

Cat. Num. AAA2009683

• Gene Names: Casp3; CC3; AC-3; Lice; Yama; mldy; CPP32; SCA-1; CASP-3; CPP-32; Apopain; Caspase-3; A830040C14Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Caspase 3 (CASP3); Recombinant Caspase 3 (CASP3); CASP3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SG IYLDSSYKMD YPEMGICIII NNKNFHKSTG MSSRSGTDVD AANLRETFMG LKYQVRNKND LTREDILELM DSVSKEDHSK RSSFVCVILS HGDEGVIYGT NGPVELKKLT SFFRGDYCRS LTGKPKLFII QACRGTELDCGIETD
• Sequence Length: 277

• Applicable Applications for CASP3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Ser29~Asp175 (Accession # P70677) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.8
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 549806822
• NCBI GeneID: 12367
• NCBI Accession #: NP_001271338.1
• NCBI GenBank Nucleotide #: NM_001284409.1
• UniProt Accession #: P70677; O08668; Q8CHV5; Q9QWI4
• Molecular Weight: 20.3kDa
• NCBI Official Full Name: caspase-3
• NCBI Official Synonym Full Names: caspase 3
• NCBI Official Symbol: Casp3
• NCBI Official Synonym Symbols: CC3; AC-3; Lice; Yama; mldy; CPP32; SCA-1; CASP-3; CPP-32; Apopain; Caspase-3; A830040C14Rik
• NCBI Protein Information: caspase-3; protein Yama; cysteine protease CPP32; SREBP cleavage activity 1; caspase 3, apoptosis related cysteine protease
• UniProt Protein Name: Caspase-3
• Protein Family: Caspase
• UniProt Gene Name: Casp3
• UniProt Synonym Gene Names: Cpp32; CASP-3; CPP-32; SCA-1
• UniProt Entry Name: CASP3_MOUSE

Uniprot Description

CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family.

Protein type: Protease; Apoptosis; Motility/polarity/chemotaxis; EC 3.4.22.56

Cellular Component: cytoplasm; cytosol; nucleus; lipid raft

Molecular Function: cyclin-dependent protein kinase inhibitor activity; peptidase activity; phospholipase A2 activator activity; protein binding; protease binding; hydrolase activity; cysteine-type endopeptidase activity; death receptor binding; protein complex binding; aspartic-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: positive regulation of catalytic activity; positive regulation of apoptosis; apoptosis; negative regulation of activated T cell proliferation; heart development; negative regulation of B cell proliferation; proteolysis; negative regulation of cell cycle; neuron differentiation; sensory perception of sound; learning and/or memory; B cell homeostasis; positive regulation of neuron apoptosis; response to glucose stimulus; response to wounding; erythrocyte differentiation; T cell homeostasis; response to UV; release of cytochrome c from mitochondria; cell fate commitment; negative regulation of cyclin-dependent protein kinase activity; keratinocyte differentiation; neuron apoptosis; response to hydrogen peroxide; induction of apoptosis via death domain receptors; protein processing; induction of apoptosis by oxidative stress; response to DNA damage stimulus; negative regulation of apoptosis