Human alpha2-Antiplasmin ELISA Kit | alpha2-AP elisa kit

Human alpha2-Antiplasmin ELISA Kit

Cat. Num. AAA765756

• Gene Names: SERPINF2; AAP; API; PLI; A2AP; ALPHA-2-PI
• Reactivity: Human
• Synonyms: alpha2-Antiplasmin; Human alpha2-Antiplasmin ELISA Kit; alpha2-AP/SERPINF2/Serpin F2/alpha 2-Antiplasmin/Alpha-2-AP/Alpha-2-PI/Alpha-2-plasmin inhibitor/pigment epithelium derived factor; a2-Antiplasmin; alpha2-AP elisa kit

• Reactivity: Human
• Sequence Length: 427

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 3.125-200ng/ml
• Sensitivity: 1.875ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for alpha2-AP elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human alpha2- AP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human alpha2-AP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ApoH amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human alpha2-AP can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 260064050
• NCBI GeneID: 5345
• NCBI Accession #: NP_001159393.1
• NCBI GenBank Nucleotide #: NM_001165921.1
• Molecular Weight: 47,907 Da
• NCBI Official Full Name: alpha-2-antiplasmin isoform b
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
• NCBI Official Symbol: SERPINF2
• NCBI Official Synonym Symbols: AAP; API; PLI; A2AP; ALPHA-2-PI
• NCBI Protein Information: alpha-2-antiplasmin
• UniProt Protein Name: Alpha-2-antiplasmin
• UniProt Gene Name: SERPINF2
• UniProt Synonym Gene Names: AAP; PLI; Alpha-2-AP; Alpha-2-PI
• UniProt Entry Name: A2AP_HUMAN

NCBI Description

This gene encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

SERPINF2: Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin. Defects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID). APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis. Belongs to the serpin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: cell surface; extracellular region; extracellular space; fibrinogen complex

Molecular Function: endopeptidase inhibitor activity; protease binding; protein binding; protein homodimerization activity; serine-type endopeptidase inhibitor activity

Biological Process: acute-phase response; blood coagulation; blood vessel morphogenesis; collagen fibril organization; fibrinolysis; negative regulation of fibrinolysis; platelet activation; platelet degranulation; positive regulation of cell differentiation; positive regulation of collagen biosynthetic process; positive regulation of JNK cascade; positive regulation of smooth muscle cell proliferation; positive regulation of stress fiber formation; positive regulation of transcription from RNA polymerase II promoter; renin-angiotensin regulation of blood vessel size; response to organic substance

Disease: Alpha-2-plasmin Inhibitor Deficiency