Rabbit CASP3 Polyclonal Antibody | anti-CASP3 antibody

CASP3 Polyclonal Antibody

Cat. Num. AAA2556400

• Gene Names: Casp3; CC3; AC-3; Lice; Yama; mldy; CPP32; SCA-1; CASP-3; CPP-32; Caspase-3; A830040C14Rik
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: CASP3; Polyclonal Antibody; CASP3 Polyclonal Antibody; A830040C14Rik; Apopain; CASP-3; Casp3a; Caspase 3; apoptosis-related cysteine peptidase; apoptosis-related cysteine protease; apoptosis-related cysteine protease a; Caspase-3 subunit p12; CC3; CPP-32; CPP32; CPP32B; Cysteine protease CPP32; EC 3.4.22.56; LICE; mldy; OTTHUMP00000165052; OTTHUMP00000165053; OTTHUMP00000165054; PARP cleavage protease; Procaspase3; Protein Yama; SCA 1; SCA-1; SREBP cleavage activity 1; Yama; anti-CASP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 100 mug/ml BSA and 50% glycerol.
• Concentration: 500 mug/mL (varies by lot)
• Sequence Length: 277

• Applicable Applications for anti-CASP3 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:500-1:1000
• Immunogen: KLH conjugated synthetic peptide corresponding to Mouse caspase3
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded A549 in Human breast using CASP3 Polyclonal Antibody at dilution of 1:400.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded A549 in Mouse lung using CASP3 Polyclonal Antibody at dilution of 1:400.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded A549 in Mouse Subcutaneous tumor using CASP3 Polyclonal Antibody at dilution of 1:400.)

Related Product Information for anti-CASP3 antibody

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs MW of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.

Product Categories/Family for anti-CASP3 antibody

Cancer; Cell Biology; Metabolism; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 549806822
• NCBI GeneID: 12367
• NCBI Accession #: NP_001271338.1
• NCBI GenBank Nucleotide #: NM_001284409.1
• UniProt Accession #: P70677; O08668; Q8CHV5; Q9QWI4
• Molecular Weight: 31,475 Da
• NCBI Official Full Name: caspase-3
• NCBI Official Synonym Full Names: caspase 3
• NCBI Official Symbol: Casp3
• NCBI Official Synonym Symbols: CC3; AC-3; Lice; Yama; mldy; CPP32; SCA-1; CASP-3; CPP-32; Caspase-3; A830040C14Rik
• NCBI Protein Information: caspase-3
• UniProt Protein Name: Caspase-3
• Protein Family: Caspase
• UniProt Gene Name: Casp3
• UniProt Synonym Gene Names: Cpp32; CASP-3; CPP-32; SCA-1

NCBI Description

This gene encodes a protein that belongs to a highly conserved family of cysteinyl aspartate-specific proteases that function as essential regulators of programmed cell death through apoptosis. Members of this family contain an N-terminal pro-domain and require cleavage at specific aspartate residues to become mature. The protein encoded by this gene belongs to a subgroup of cysteinyl aspartate-specific proteases that are activated by initiator caspases and that perform the proteolytic cleavage of apoptotic target proteins. Mice defective for this gene exhibit a variety of phenotypes including reduced neuronal apoptosis resulting in hyperplasias, hearing loss, attenuated osteogenic differentiation of bone marrow stromal stem cells, and pre- and post-natal lethality. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.