Mouse anti-Human RASEF Monoclonal Antibody | anti-RASEF antibody

RASEF (Ras and EF-hand Domain-containing Protein, Ras-related Protein Rab-45, RAB45) APC

Cat. Num. AAA6138632

• Gene Names: RASEF; RAB45
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RASEF; Monoclonal Antibody; RASEF (Ras and EF-hand Domain-containing Protein; Ras-related Protein Rab-45; RAB45) APC; FLJ31614; anti-RASEF antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 2D5
• Specificity: Recognizes human RASEF.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 2770

• Applicable Applications for anti-RASEF antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 3ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant protein corresponding to aa631-740 from human RASEF (AAH23566) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: NIREWVDMIEDAAHETVPIMLVGNKADIRDTAATEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSRSITNLTGTNSKKSPQMKNCCNG
• Conjugate: APC
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (38.1kD).)

Western Blot (WB)

(RASEF monoclonal antibody, Western Blot analysis of RASEF expression in K-562.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to RASEF on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 3ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged RASEF is ~0.3ng/ml as a capture antibody.)

Product Categories/Family for anti-RASEF antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 23270807
• NCBI GeneID: 158158
• NCBI Official Full Name: Homo sapiens RAS and EF-hand domain containing, mRNA
• NCBI Official Synonym Full Names: RAS and EF-hand domain containing
• NCBI Official Symbol: RASEF
• NCBI Official Synonym Symbols: RAB45
• NCBI Protein Information: ras and EF-hand domain-containing protein
• Protein Family: Ras and EF-hand domain-containing protein

NCBI Description

This gene is a member of the Rab family of GTPases that are involved in regulation of membrane traffic. The encoded protein contains an N-terminal EF-hand domain, a coiled-coil motif and a C-terminal Rab domain. A potential role as tumor suppressor has been indicated for this gene. [provided by RefSeq, Nov 2012]