Caspase-1 Recombinant Protein | CASP1 recombinant protein

Caspase-1, human recombinant

Cat. Num. AAA844389

• Gene Names: CASP1; ICE; P45; IL1BC
• Applications: SDS-Page
• Synonyms: Caspase-1; human recombinant; CASP-1; Interleukin-1 beta convertase; IL-1BC; IL-1 beta-converting enzyme; p45; CASP1 recombinant protein

• Host: E Coli
• Form/Format: Lyophilized powder with additives
• Sequence Length: 404

• Applicable Applications for CASP1 recombinant protein: SDS-PAGE; HPLC
• Application Notes: Active caspase-1 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity. ; For a complete caspase-1 assay protocol, please refer to Caspase-1 Fluorometric or Colorimetric Assay Kits (Cat. No. please inquire)
• Reconstitution: Reconstitute to 1 unit per ul in water.
• Specific Activity: > 5000 units/mg
• Unit Definition: One unit of the recombinant caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate YVAD-pNA (pNA: pnitroanaline) per hour at 37 degree C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: The lyophilized caspase-1 is stable for 1 year at –70 °C. Following reconstitution in water, the enzyme should be aliquoted and immediately stored at –70 °C. Avoid multiple freeze/thaw cycles as activity might decrease.

SDS-PAGE

Related Product Information for CASP1 recombinant protein

Background: Caspase-1 (also know as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kD) and small (10 kD) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form. The recombinant active human caspase-1 was expressed in E. coli. The active caspase-1 preferentially cleaves caspase-1 substrates (e.g., YVAD-AFC or YVAD-pNA) and is routinely tested at MyBioSource for its ability to enzymatically cleave these two substrates Ac-YVAD-pNA or Ac-YVAD-AFC (1103-200). The rh-Caspase has an N-term His-tag and corresponds to amino acids 120-404 of Caspase-1, gene accession# NP_150634.1.

NCBI and Uniprot Product Information

• NCBI GI #: 15431328
• NCBI GeneID: 834
• NCBI Accession #: NP_150634.1
• NCBI GenBank Nucleotide #: NM_033292.3
• NCBI Official Full Name: caspase-1 isoform alpha
• NCBI Official Synonym Full Names: caspase 1, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP1
• NCBI Official Synonym Symbols: ICE; P45; IL1BC
• NCBI Protein Information: caspase-1
• UniProt Protein Name: Caspase-1
• Protein Family: Caspase
• UniProt Gene Name: CASP1
• UniProt Synonym Gene Names: IL1BC; IL1BCE; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme
• UniProt Entry Name: CASP1_HUMAN

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]

Uniprot Description

CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Interacts with CARD17/INCA and CARD18. Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. Specifically inhibited by the cowpox virus Crma protein. Belongs to the peptidase C14A family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; EC 3.4.22.36; Protease

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: cytoplasm; extracellular region; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; endopeptidase activity; caspase activator activity

Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; membrane hyperpolarization; interleukin-1 beta production; response to lipopolysaccharide; mitochondrial depolarization; positive regulation of interleukin-1 beta secretion; signal transduction; proteolysis; response to ATP; positive regulation of interleukin-1 alpha secretion; regulation of inflammatory response; response to hypoxia; innate immune response; regulation of autophagy