CART Recombinant Protein | CARTPT recombinant protein

CART Protein, Human, Recombinant

Cat. Num. AAA8121350

• Gene Names: CARTPT; CART
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CART; CART Protein; Human; Recombinant; Human CART/CARTPT Protein; CART prepropeptide; CARTPT recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu116

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 28
• Tag: C-cleavage
• Protein Construction: A DNA sequence encoding the human CARTPT (NP_004282.1)(Met1-Leu116) was expressed with five amino acids (DDDDK) at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CARTPT recombinant protein

Background: In RNAseq data from human islet donors, CARTPT expression levels correlated with insulin, exocytosis genes and key beta-cell transcription factors. Insufficient insulin release and hyperglucagonaemia are culprits in type 2 diabetes. Cocaine- and amphetamine-regulated transcript (CART, encoded by Cartpt) affects islet hormone secretion and beta cell survival in vitro in rats, and Cart (-/-) mice have diminished insulin secretion. Established a potential role for cocaine and amphetamine regulated transcript (CARTPT) in dominant follicle selection in cattle. CARTPT expression is elevated in subordinate versus dominant follicles, and treatment with the mature form of the CARTPT peptide (CART) decreases follicle-stimulating hormone (FSH)-stimulated granulosa cell estradiol production in vitro and follicular fluid estradiol and granulosa cell CYP19A1 mRNA in vivo.

NCBI and Uniprot Product Information

• NCBI GI #: 4757910
• NCBI GeneID: 9607
• NCBI Accession #: NP_004282.1
• NCBI GenBank Nucleotide #: NM_004291.3
• UniProt Accession #: Q16568; Q6FG92
• Molecular Weight: 12,829 Da
• NCBI Official Full Name: cocaine- and amphetamine-regulated transcript protein preproprotein
• NCBI Official Synonym Full Names: CART prepropeptide
• NCBI Official Symbol: CARTPT
• NCBI Official Synonym Symbols: CART
• NCBI Protein Information: cocaine- and amphetamine-regulated transcript protein
• UniProt Protein Name: Cocaine- and amphetamine-regulated transcript protein
• Protein Family: Cartilage acidic protein
• UniProt Gene Name: CARTPT
• UniProt Synonym Gene Names: CART
• UniProt Entry Name: CART_HUMAN

NCBI Description

This gene encodes a preproprotein that is proteolytically processed to generate multiple biologically active peptides. These peptides play a role in appetite, energy balance, maintenance of body weight, reward and addiction, and the stress response. Expression of a similar gene transcript in rodents is upregulated following administration of cocaine and amphetamine. Mutations in this gene are associated with susceptibility to obesity in humans. [provided by RefSeq, Feb 2016]

Uniprot Description

CARTPT: Satiety factor closely associated with the actions of leptin and neuropeptide y; this anorectic peptide inhibits both normal and starvation-induced feeding and completely blocks the feeding response induced by neuropeptide Y and regulated by leptin in the hypothalamus. It promotes neuronal development and survival in vitro. Belongs to the CART family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide; Hormone

Chromosomal Location of Human Ortholog: 5q13.2

Cellular Component: extracellular space; secretory granule

Molecular Function: protein binding

Biological Process: activation of MAPKK activity; adult feeding behavior; cell glucose homeostasis; cell-cell signaling; cellular response to starvation; circadian regulation of gene expression; G-protein coupled receptor protein signaling pathway; negative regulation of appetite; negative regulation of bone resorption; negative regulation of osteoclast differentiation; neuropeptide signaling pathway; positive regulation of blood pressure; positive regulation of epinephrine secretion; positive regulation of transmission of nerve impulse; regulation of insulin secretion; signal transduction; synaptic transmission

Disease: Obesity