Rabbit anti-Mouse CARTPT Polyclonal Antibody | anti-CARTPT antibody

CARTPT, NT (CARTPT, CART, Cocaine-and amphetamine-regulated transcript protein, CART(1-39), CART(42-89)) (FITC)

Cat. Num. AAA6280829

• Gene Names: CARTPT; CART
• Reactivity: Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CARTPT; Polyclonal Antibody; NT (CARTPT; CART; Cocaine-and amphetamine-regulated transcript protein; CART(1-39); CART(42-89)) (FITC); CART(42-89); anti-CARTPT antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CARTPT antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CARTPT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-55 amino acids from the N-terminal region of human CARTPT.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CARTPT Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the CARTPT antibody detected the CARTPT protein (arrow).)

Immunohistochemistry (IHC)

(CARTPT Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CARTPT Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CARTPT antibody

This gene encodes a secreted protein which is processed by prohormone/proprotein convertases to produce smaller, biologically active peptides. Expression of the transcript for this gene is regulated by certain drugs such as cocaine, and the encoded protein is thought to be involved in the regulation of appetite and stress. Mutations in this gene are associated with susceptibility to obesity.

Product Categories/Family for anti-CARTPT antibody

Antibodies; Proteins

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Busto, A., et al. Prog. Neuropsychopharmacol. Biol. Psychiatry 34(6):834-836(2010). Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010): Rigoli, L., et al. Acta Paediatr. 99(5):722-726(2010). Vasseur, F., et al. J. Hum. Genet. 55(4):227-231(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4757910
• NCBI GeneID: 9607
• NCBI Accession #: NP_004282.1
• NCBI GenBank Nucleotide #: NM_004291.3
• UniProt Accession #: Q16568; Q6FG92
• Molecular Weight: 12,829 Da
• NCBI Official Full Name: cocaine- and amphetamine-regulated transcript protein preproprotein
• NCBI Official Synonym Full Names: CART prepropeptide
• NCBI Official Symbol: CARTPT
• NCBI Official Synonym Symbols: CART
• NCBI Protein Information: cocaine- and amphetamine-regulated transcript protein
• UniProt Protein Name: Cocaine- and amphetamine-regulated transcript protein
• Protein Family: Cocaine- and amphetamine-regulated transcript protein
• UniProt Gene Name: CARTPT
• UniProt Synonym Gene Names: CART
• UniProt Entry Name: CART_HUMAN

NCBI Description

This gene encodes a preproprotein that is proteolytically processed to generate multiple biologically active peptides. These peptides play a role in appetite, energy balance, maintenance of body weight, reward and addiction, and the stress response. Expression of a similar gene transcript in rodents is upregulated following administration of cocaine and amphetamine. Mutations in this gene are associated with susceptibility to obesity in humans. [provided by RefSeq, Feb 2016]

Uniprot Description

CARTPT: Satiety factor closely associated with the actions of leptin and neuropeptide y; this anorectic peptide inhibits both normal and starvation-induced feeding and completely blocks the feeding response induced by neuropeptide Y and regulated by leptin in the hypothalamus. It promotes neuronal development and survival in vitro. Belongs to the CART family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide; Hormone

Chromosomal Location of Human Ortholog: 5q13.2

Cellular Component: extracellular space; secretory granule

Molecular Function: protein binding

Biological Process: activation of MAPKK activity; adult feeding behavior; cell glucose homeostasis; cell-cell signaling; cellular response to starvation; circadian regulation of gene expression; G-protein coupled receptor protein signaling pathway; negative regulation of appetite; negative regulation of bone resorption; negative regulation of osteoclast differentiation; neuropeptide signaling pathway; positive regulation of blood pressure; positive regulation of epinephrine secretion; positive regulation of transmission of nerve impulse; regulation of insulin secretion; signal transduction; synaptic transmission

Disease: Obesity