Rabbit anti-Human KIRREL Polyclonal Antibody | anti-KIRREL antibody

KIRREL, CT (KIRREL, KIRREL1, NEPH1, Kin of IRRE-like protein 1, Kin of irregular chiasm-like protein 1, Nephrin-like protein 1) (APC)

Cat. Num. AAA6313447

• Gene Names: KIRREL; NEPH1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KIRREL; Polyclonal Antibody; CT (KIRREL; KIRREL1; NEPH1; Kin of IRRE-like protein 1; Kin of irregular chiasm-like protein 1; Nephrin-like protein 1) (APC); Nephrin-like protein 1; anti-KIRREL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-KIRREL antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KIRREL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 505-534 amino acids from the C-terminal region of human KIRREL.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(KIRREL Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the KIRREL antibody detected the KIRREL protein (arrow).)

Related Product Information for anti-KIRREL antibody

NEPH1 is a member of the nephrin-like protein family, which includes NEPH2 (MIM 607761) and NEPH3 (MIM 607762). The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2; MIM 604766), and the genes are expressed in kidney podocytes, cells involved in ensuring size-and charge-selective ultrafiltration (Sellin et al., 2003 [PubMed 12424224]).

Product Categories/Family for anti-KIRREL antibody

Antibodies; Nephrin

References

Machuca, E., et al. J. Am. Soc. Nephrol. 21(7):1209-1217(2010). Wagner, M.C., et al. J. Biol. Chem. 283(51):35579-35589(2008). Hartleben, B., et al. J. Biol. Chem. 283(34):23033-23038(2008). Harita, Y., et al. J. Biol. Chem. 283(14):9177-9186(2008). Ihalmo, P., et al. Diabetologia 51(1):86-90(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 166295173
• NCBI GeneID: 55243
• NCBI Accession #: NP_060710.3
• NCBI GenBank Nucleotide #: NM_018240.6
• UniProt Accession #: Q96J84; Q5W0F8; Q5XKC6; Q7Z696; Q7Z7N8; Q8TB15; Q9H9N1; Q9NVA5
• Molecular Weight: 72,360 Da
• NCBI Official Full Name: kin of IRRE-like protein 1 isoform 1
• NCBI Official Synonym Full Names: kin of IRRE like (Drosophila)
• NCBI Official Symbol: KIRREL
• NCBI Official Synonym Symbols: NEPH1
• NCBI Protein Information: kin of IRRE-like protein 1
• UniProt Protein Name: Kin of IRRE-like protein 1
• Protein Family: Kin of IRRE-like protein
• UniProt Gene Name: KIRREL
• UniProt Synonym Gene Names: KIRREL1; NEPH1
• UniProt Entry Name: KIRR1_HUMAN

NCBI Description

NEPH1 is a member of the nephrin-like protein family, which includes NEPH2 (MIM 607761) and NEPH3 (MIM 607762). The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2; MIM 604766), and the genes are expressed in kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration (Sellin et al., 2003 [PubMed 12424224]).[supplied by OMIM, Mar 2008]

Uniprot Description

Function: Plays a significant role in the normal development and function of the glomerular permeability. Signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1

By similarity.

Subunit structure: Interacts with TJP1/ZO-1 and with NPHS2/podocin (via the C-terminus). Interacts with NPHS1/nephrin (via the Ig-like domains); this interaction is dependent on KIRREL glycosylation. Homodimer (via the Ig-like domains). Interacts when tyrosine-phosphorylated with GRB2

By similarity.

Subcellular location: Cell membrane; Single-pass type I membrane protein

Potential. Note: Predominantly located at podocyte slit diaphragm.

Tissue specificity: Abundantly expressed in kidney. Specifically expressed in podocytes of kidney glomeruli. Ref.1

Post-translational modification: Phosphorylation probably regulates the interaction with NSH2. Phosphorylated at Tyr-605 and Tyr-606 by FYN, leading to GRB2 binding

By similarity.N-glycosylated

By similarity.

Sequence similarities: Belongs to the immunoglobulin superfamily.Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

Sequence caution: The sequence AAP59845.1 differs from that shown. Reason: Frameshift at position 19. The sequence BAA91850.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB14192.1 differs from that shown. Reason: Erroneous initiation.