Western Blot (WB)
(Western BlotPositive WB detected in: Jurkat whole cell lysateAll lanes: CARMIL2 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 155, 149 kDaObserved band size: 155 kDa)
Rabbit anti-Human CARMIL2 Polyclonal Antibody | anti-CARMIL2 antibody
CARMIL2 Antibody
Protein G Purified
Protein G Purified
IP: 1:200-1:2000
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Western Blot (WB)
(Western BlotPositive WB detected in: Jurkat whole cell lysateAll lanes: CARMIL2 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 155, 149 kDaObserved band size: 155 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in: Jurkat whole cell lysateAll lanes: CARMIL2 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 155, 149 kDaObserved band size: 155 kDa)
Immunoprecipitation (IP)
(Immunoprecipitating CARMIL2 in Jurkat whole cell lysateLane 1: Rabbit control IgG instead of CSB-PA739364LA01HU in Jurkat whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-PA739364LA01HU (6ug) + Jurkat whole cell lysate (500ug)Lane 3: Jurkat whole cell lysate (20ug))
Immunoprecipitation (IP)
(Immunoprecipitating CARMIL2 in Jurkat whole cell lysateLane 1: Rabbit control IgG instead of CSB-PA739364LA01HU in Jurkat whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-PA739364LA01HU (6ug) + Jurkat whole cell lysate (500ug)Lane 3: Jurkat whole cell lysate (20ug))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the CARMIL (capping protein, Arp2/3, myosin-I linker) family of proteins. The encoded protein interacts with and negatively regulates the heterodimeric capping protein and promotes cell migration. Reduced expression of this gene has been observed in human psoriasis patients. Mutations in this gene cause a human immunodeficiency syndrome characterized by smooth muscle tumors and impaired T-cell function. [provided by RefSeq, May 2017]
Uniprot Description
Cell membrane-cytoskeleton-associated protein that plays a role in the regulation of actin polymerization at the barbed end of actin filaments. Prevents F-actin heterodimeric capping protein (CP) activity at the leading edges of migrating cells, and hence generates uncapped barbed ends and enhances actin polymerization (PubMed:26466680). Plays a role in cell protrusion formations; involved in cell polarity, lamellipodial assembly, membrane ruffling and macropinosome formations (PubMed:19846667, PubMed:26578515, PubMed:26466680). Involved as well in cell migration and invadopodia formation during wound healing (PubMed:19846667, PubMed:26578515, PubMed:26466680). Required for CD28-mediated stimulation of NF-kappa-B signaling, involved in naive T cells activation, maturation into T memory cells, and differentiation into T helper and T regulatory cells (PubMed:27647349, PubMed:27647348, PubMed:28112205).
Research Articles on CARMIL2
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Product Notes
The CARMIL2 carmil2 (Catalog #AAA7114050) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CARMIL2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CARMIL2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 1:200-1:2000. Researchers should empirically determine the suitability of the CARMIL2 carmil2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CARMIL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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