Mouse anti-Mouse, Human RLTPR Monoclonal Antibody | anti-RLTPR antibody

Anti-RLTPR Purified

Cat. Num. AAA1800611

• Gene Names: CARMIL2; IMD58; RLTPR; LRRC16C; CARMIL2b
• Reactivity: Mouse, Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein-A Affinity Chromatography
• Synonyms: RLTPR; Monoclonal Antibody; Anti-RLTPR Purified; capping protein regulator and myosin 1 linker 2; CARMIL2; LRRC16C; anti-RLTPR antibody

• Host: Mouse
• Reactivity: Mouse, Human
• Clonality: Monoclonal
• Isotype: IgG1 kappa
• Clone Number: EM-53
• Specificity: The mouse monoclonal antibody EM-53 recognizes RLTPR / CARMIL2, an intracellular protein playing a role in actin filament elongation.
• Purity/Purification: Purified by Protein-A Affinity Chromatography
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1435

• Applicable Applications for anti-RLTPR antibody: Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: FC/FACS: QC tested
• Immunogen: Murine RLTPR
• Preparation and Storage: Store at 2-8 degree C.

Flow Cytometry (FC/FACS)

(Separation of RLTPR transfected cells stained using anti-human RLTPR (EM-53) purified antibody (GAM FITC, concentration in sample 9 ug/ml, red-filled) from RLTPR transfected cells stained using mouse IgG1 isotype control (MOPC-21) purified antibody (GAM FITC, concentration in sample 9 ug/ml, black-dashed) in flow cytometry analysis (surface staining).)

Related Product Information for anti-RLTPR antibody

RLTPR / CARMIL2 (RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing; capping protein regulator and myosin 1 linker 2), also known as LRRC16C, is a cytosolic protein, which with high affinity binds CAPZA2 (capping protein muscle actin Z-line alpha 2) and decreases CAPZA2 affinity for actin barbed ends. RLTPR / CARMIL2 increases the rate of actin filament elongation from seeds in the presence of CAPZA2, however, seems unable to nucleate filaments. Its interaction with CAPZA2 is essential for lamellipodial protrusion and cell translocation. RLTPR / CARMIL2 is crutial for T cell costimulation via CD28 and this property seems to be independent on its actin-uncapping function. The lack of functional RLTPR / CARMIL2 molecules impeded the differentiation toward Th1 and Th17 fates of both human and murine CD4+ T cells and leads to combined immunodeficiency. Expression of RLTPR / CARMIL2 was also detected in human and murine B cells, but it seems not to be involved in BCR-mediated signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 66392157
• NCBI GeneID: 146206
• NCBI Accession #: NP_001013860.1
• NCBI GenBank Nucleotide #: NP_001013860.1
• UniProt Accession #: Q6F5E8
• NCBI Official Full Name: capping protein, Arp2/3 and myosin-I linker protein 2 isoform 1
• NCBI Official Synonym Full Names: capping protein regulator and myosin 1 linker 2
• NCBI Official Symbol: CARMIL2
• NCBI Official Synonym Symbols: IMD58; RLTPR; LRRC16C; CARMIL2b
• NCBI Protein Information: capping protein, Arp2/3 and myosin-I linker protein 2
• UniProt Protein Name: Leucine-rich repeat-containing protein 16C
• UniProt Gene Name: RLTPR
• UniProt Synonym Gene Names: CARMIL2b; LRRC16C
• UniProt Entry Name: LR16C_HUMAN

NCBI Description

This gene encodes a member of the CARMIL (capping protein, Arp2/3, myosin-I linker) family of proteins. The encoded protein interacts with and negatively regulates the heterodimeric capping protein and promotes cell migration. Reduced expression of this gene has been observed in human psoriasis patients. Mutations in this gene cause a human immunodeficiency syndrome characterized by smooth muscle tumors and impaired T-cell function. [provided by RefSeq, May 2017]