Human SCF PicoKine ELISA Kit | KITLG elisa kit

Human SCF PicoKine ELISA Kit

Cat. Num. AAA177566

• Gene Names: KITLG; SF; MGF; SCF; DCUA; FPH2; FPHH; KL-1; Kitl; SHEP7
• Reactivity: Human
• Synonyms: SCF PicoKine; Human SCF PicoKine ELISA Kit; Kit ligand; C kit ligand; Ckit ligand; Kitl; KITLG; KL 1; KL1; Mast cell growth factor; MGF; SF; SHEP7; Steel factor; Stem cell factor; Stem cell factor precursor; Stem Cell Factor (SCF); KITLG elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human SCF
• Sequence Length: 273

• Assay Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of human SCF in cell culture supernates, serum and plasma (heparin, EDTA, citrate).
• Immunogen: E Coli, E26-A190
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human SCF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA, citrate) to each empty well. It is recommended that each human SCF standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|115|450|1558|120|400|1889; Standard Deviation|5.75|23.40|91.92|7.32|26.40|122.78; CV(%)|5.0|5.2|5.9|6.1|6.6|6.5
• Sample Data: Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000; O.D.|0.004|0.102|0.175|0.333|0.559|1.021|1.672|2.054
• Anticoagulant: Heparin, EDTA or Citrate
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human SCF PicoKine ELISA Kit standard curve )

Related Product Information for KITLG elisa kit

Principle of the assay: Human SCF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SCF has been precoated onto 96-well plates. Standards (E Coli, E26-A190) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SCF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human SCF amount of sample captured in plate.

Background: Stem Cell Factor (also known as SCF, kit-ligand, KL, or steel factor) is a cytokine that binds to the c-Kit receptor (CD117). It is mapped to 12q21.32. SCF was primarily expressed by perivascular cells throughout the bone marrow, and it plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF can exist both as a transmembrane protein and a soluble protein. This gene plays an important role in the hematopoiesis during embryonic development. During development, the presence of the SCF also plays an important role in the localization of melanocytes, cells that produce melanin and control pigmentation. In addition to it, SCF can promote mast cell adhesion, migration, proliferation, and survival. It also promotes the release of histamine and tryptase, which are involved in the allergic response.

NCBI and Uniprot Product Information

• NCBI GI #: 4505175
• NCBI GeneID: 4254
• NCBI Accession #: NP_000890.1
• NCBI GenBank Nucleotide #: NM_000899.4
• UniProt Accession #: P21583; Q16487; Q68DZ2; Q7M4N8; Q9UQK7; A0AV09; A8K2Q4; B7ZLM4
• Molecular Weight: 26,667 Da
• NCBI Official Full Name: kit ligand isoform b
• NCBI Official Synonym Full Names: KIT ligand
• NCBI Official Symbol: KITLG
• NCBI Official Synonym Symbols: SF; MGF; SCF; DCUA; FPH2; FPHH; KL-1; Kitl; SHEP7
• NCBI Protein Information: kit ligand
• UniProt Protein Name: Kit ligand
• Protein Family: Kit ligand
• UniProt Gene Name: KITLG
• UniProt Synonym Gene Names: MGF; SCF; MGF; SCF; sKITLG
• UniProt Entry Name: SCF_HUMAN

NCBI Description

This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SCF: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. Belongs to the SCF family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: cytoplasm; cytoskeleton; extracellular region; extracellular space; integral to membrane; plasma membrane

Molecular Function: cytokine activity; growth factor activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; Ras guanyl-nucleotide exchange factor activity; stem cell factor receptor binding

Biological Process: cell adhesion; cell proliferation; embryonic hemopoiesis; germ cell programmed cell death; male gonad development; MAPKKK cascade; negative regulation of mast cell apoptosis; neural crest cell migration; ovarian follicle development; phosphoinositide phosphorylation; phosphoinositide-mediated signaling; positive regulation of DNA replication; positive regulation of GTPase activity; positive regulation of leukocyte migration; positive regulation of MAP kinase activity; positive regulation of melanocyte differentiation; positive regulation of myeloid leukocyte differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of Ras protein signal transduction; regulation of phosphoinositide 3-kinase cascade; signal transduction

Disease: Hyperpigmentation, Familial Progressive, 2; Skin/hair/eye Pigmentation, Variation In, 7