Western Blot (WB)
(Anti-CARHSP1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 293T Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:16 kDaObserved band size:22 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human CARHSP1 Polyclonal Antibody | anti-CARHSP1 antibody
Anti-CARHSP1 Antibody, Rabbit Polyclonal
Gene Names
CARHSP1; CSDC1; CRHSP24; CRHSP-24
Reactivity
Human
Applications
Western Blot, ELISA, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
CARHSP1; Polyclonal Antibody; Anti-CARHSP1 Antibody; Rabbit Polyclonal; CARHSP1 Antibody; Rabbit PAb; Antigen Affinity Purified; calcium regulated heat stable protein 1; 24kDa; Anti-CRHSP-24 Antibody; Anti-CSDC1 Antibody; anti-CARHSP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CARHSP1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CARHSP1 antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
IP: 0.5-2uL/mg of lysate
ELISA: 1:5000-1:10,000
IP: 0.5-2uL/mg of lysate
Immunogen
Recombinant Human CARHSP1 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human CARHSP1 (rh CARHSP1). CARHSP1 specific IgG was purified by Human CARHSP1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-CARHSP1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 293T Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:16 kDaObserved band size:22 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-CARHSP1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 293T Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:16 kDaObserved band size:22 kDa(We are unsure as to the identity of these extra bands.))
Immunoprecipitation (IP)
(CARHSP1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate2 uL anti-CARHSP1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CARHSP1 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size: 22 kDa)
Immunoprecipitation (IP)
(CARHSP1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate2 uL anti-CARHSP1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CARHSP1 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size: 22 kDa)
Related Product Information for anti-CARHSP1 antibody
CARHSP1 is a biomarker for diabetic complications. Adenovirus-mediated CARHSP1 overexpression and siRNA-mediated knockdown experiments were performed to characterize the role of CARHSP1 in the regulation of gluconeogenic gene expression. CARHSP1 is regulated by nutrient status in the liver and functions at the transcriptional level to negatively regulate gluconeogenic genes, including the glucose-6-phosphatase catalytic subunit (G6Pc) and phosphoenolpyruvate carboxykinase 1 (PEPCK1). In addition, it is found that CARHSP1 can physically interact with peroxisome proliferator-activated receptor-alpha (PPARalpha) and inhibit its transcriptional activity. Both pharmacological and genetic ablations of PPARalpha attenuate the inhibitory effect of CARHSP1 on gluconeogenic gene expression in hepatocytes.
References
Wistow G. et al., 2002, Mol Vis. 8: 205-20. Wishart MJ. et al., 2002, Proc Natl Acad Sci. 99 (4): 2112-7. Groblewski GE. et al., 1998, J Biol Chem. 273 (35): 22738-44. Fan Y. et al., 2011, J Biol Chem. 286 (47): 40584-94.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18.0 kDa (167aa), confirmed by MALDI-TOF
NCBI Official Full Name
calcium-regulated heat-stable protein 1
NCBI Official Synonym Full Names
calcium regulated heat stable protein 1
NCBI Official Symbol
CARHSP1
NCBI Official Synonym Symbols
CSDC1; CRHSP24; CRHSP-24
NCBI Protein Information
calcium-regulated heat-stable protein 1; calcium-regulated heat stable protein 1
UniProt Protein Name
Calcium-regulated heat-stable protein 1
Protein Family
UniProt Gene Name
CARHSP1
UniProt Synonym Gene Names
CRHSP-24
Uniprot Description
Binds mRNA and regulates the stability of target mRNA. Binds single-stranded DNA (in vitro).
Research Articles on CARHSP1
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Product Notes
The CARHSP1 carhsp1 (Catalog #AAA8107318) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CARHSP1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CARHSP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IP: 0.5-2uL/mg of lysate. Researchers should empirically determine the suitability of the CARHSP1 carhsp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CARHSP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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