Goat Adiponutrin Polyclonal Antibody | anti-PNPLA3 antibody

Adiponutrin (PNPLA3, Patatin-like Phospholipase Domain-containing Protein 3, Acylglycerol O-acyltransferase, Calcium-independent Phospholipase A2-epsilon, iPLA2-epsilon, Pnpla3, Adpn)

Cat. Num. AAA6009217

• Gene Names: PNPLA3; ADPN; C22orf20; iPLA(2)epsilon
• Reactivity: Canine, Human, Mouse, Porcine, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Adiponutrin; Polyclonal Antibody; Adiponutrin (PNPLA3; Patatin-like Phospholipase Domain-containing Protein 3; Acylglycerol O-acyltransferase; Calcium-independent Phospholipase A2-epsilon; iPLA2-epsilon; Pnpla3; Adpn); Anti -Adiponutrin (PNPLA3; anti-PNPLA3 antibody

• Host: Goat
• Reactivity: Canine, Human, Mouse, Porcine, Rat
• Clonality: Polyclonal
• Specificity: Recognizes mouse PNPLA3. Species sequence homology: Canine, human, porcine and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-PNPLA3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Immunohistochemistry and Western Blot.; Dilution: ELISA: 1:128,000; Immunohistochemistry (Paraffin): 4-6ug/ml; Western Blot: 0.05-0.2ug/ml, observed in mouse heart lysates on ~45kD bands
• Immunogen: Synthetic peptide corresponding to EHDICPKVKSTN, from mouse PNPLA3, at the internal region of the protein (NP_473429.2).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

((0.05ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-PNPLA3 antibody

Multifunctional enzyme which has both triacylglycerol lipase and acylglycerol O-acyltransferase activities.

Product Categories/Family for anti-PNPLA3 antibody

Antibodies; Abs to Enzymes; Acyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 119593731
• NCBI GeneID: 80339
• UniProt Accession #: Q9NST1; Q6P1A1; Q96CB4; B0QYI0; B2RCL3; B3KW00
• Molecular Weight: 52,865 Da
• NCBI Official Full Name: adiponutrin, isoform CRA_c
• NCBI Official Synonym Full Names: patatin-like phospholipase domain containing 3
• NCBI Official Symbol: PNPLA3
• NCBI Official Synonym Symbols: ADPN; C22orf20; iPLA(2)epsilon
• NCBI Protein Information: patatin-like phospholipase domain-containing protein 3; adiponutrin; iPLA2epsilon; iPLA2-epsilon; acylglycerol O-acyltransferase; calcium-independent phospholipase A2-epsilon
• UniProt Protein Name: Patatin-like phospholipase domain-containing protein 3
• Protein Family: Patatin-like phospholipase domain-containing protein
• UniProt Gene Name: PNPLA3
• UniProt Synonym Gene Names: ADPN; C22orf20; iPLA2-epsilon
• UniProt Entry Name: PLPL3_HUMAN

NCBI Description

The protein encoded by this gene is a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. The encoded protein, which appears to be membrane bound, may be involved in the balance of energy usage/storage in adipocytes. [provided by RefSeq, Jul 2008]

Uniprot Description

PNPLA3: Multifunctional enzyme which has both triacylglycerol lipase and acylglycerol O-acyltransferase activities. Genetic variations in PNPLA3 are a cause of susceptibility to non-alcoholic fatty liver disease type 1 (NAFLD1). A condition characterized by an accumulation of excess triglyceride in the liver, a condition known as hepatic steatosis (or fatty liver), which is associated with adverse metabolic consequences, including insulin resistance and dyslipidemia. In a subset of individuals, hepatic steatosis promotes an inflammatory response in the liver, referred to as steatohepatitis, which can progress to cirrhosis and liver cancer. NAFLD is the most common form of liver disease in Western countries. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.3; Phospholipase; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - glycerolipid; Transferase; Secondary Metabolites Metabolism - limonene and pinene degradation; Amino Acid Metabolism - phenylalanine; Membrane protein, integral; Amino Acid Metabolism - tyrosine

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: endoplasmic reticulum membrane; membrane; integral to membrane

Molecular Function: phospholipase A2 activity; triacylglycerol lipase activity; mono-olein transacylation activity; diolein transacylation activity

Biological Process: phospholipid metabolic process; triacylglycerol catabolic process; glycerophospholipid biosynthetic process; triacylglycerol biosynthetic process