Monkey Chemokine C-C-Motif Ligand 3 Like Protein 1 ELISA Kit | CCL3L1 elisa kit

Monkey Chemokine C-C-Motif Ligand 3 Like Protein 1 ELISA Kit

Cat. Num. AAA738641

• Gene Names: CCL3L1; LD78; 464.2; MIP1AP; SCYA3L; G0S19-2; SCYA3L1; D17S1718; LD78BETA; LD78-beta(1-70)
• Reactivity: Monkey
• Synonyms: Chemokine C-C-Motif Ligand 3 Like Protein 1; Monkey Chemokine C-C-Motif Ligand 3 Like Protein 1 ELISA Kit; CCL3L1 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 250-5000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This CCL3L1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey CCL3L1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL3L1 elisa kit

Principle of the Assay: CCL3L1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCL3L1 antibody and an CCL3L1-HRP conjugate. The assay sample and buffer are incubated together with CCL3L1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCL3L1 concentration since CCL3L1 from samples and CCL3L1-HRP conjugate compete for the anti-CCL3L1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCL3L1 from the sample, fewer sites are left to bind CCL3L1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCL3L1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CCL3L1 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 27477072
• NCBI GeneID: 6349
• NCBI Accession #: NP_066286.1
• NCBI GenBank Nucleotide #: NM_021006.5
• Molecular Weight: 10,161 Da
• NCBI Official Full Name: C-C motif chemokine 3-like 1
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 3-like 1
• NCBI Official Symbol: CCL3L1
• NCBI Official Synonym Symbols: LD78; 464.2; MIP1AP; SCYA3L; G0S19-2; SCYA3L1; D17S1718; LD78BETA; LD78-beta(1-70)
• NCBI Protein Information: C-C motif chemokine 3-like 1; small inducible cytokine A3-like 1; G0/G1 switch regulatory protein 19-2; tonsillar lymphocyte LD78 beta protein
• UniProt Protein Name: C-C motif chemokine 3-like 1
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL3L1
• UniProt Synonym Gene Names: D17S1718; G0S19-2; SCYA3L1; CCL3L3
• UniProt Entry Name: CL3L1_HUMAN

NCBI Description

This gene is one of several cytokine genes that are clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins that function in inflammatory and immunoregulatory processes. The protein encoded by this gene binds to several chemokine receptors, including chemokine binding protein 2 and chemokine (C-C motif) receptor 5 (CCR5). CCR5 is a co-receptor for HIV, and binding of this protein to CCR5 inhibits HIV entry. The copy number of this gene varies among individuals, where most individuals have one to six copies, and a minority of individuals have zero or more than six copies. There are conflicting reports about copy number variation of this gene and its correlation to disease susceptibility. This record represents one of two copies that are present on the ALT_REF_LOCI_2 alternate haplotype of the GRCh38 human reference genome assembly. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

CCL3L1: Chemotactic for lymphocytes and monocytes. Is a ligand for CCR1, CCR3 and CCR5. Is an inhibitor of HIV-1-infection. The processed form LD78-beta(3-70) shows a 20-fold to 30-fold higher chemotactic activity and is a very potent inhibitor of HIV-1- infection. LD78-beta(3-70) is also a ligand for CCR1, CCR3 and CCR5. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Chemokine; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21.1

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity

Biological Process: negative regulation of cell proliferation; immune response; inflammatory response

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To