SDS-Page
CARHSP1 recombinant protein
CARHSP1 Protein, Human, Recombinant (His Tag)
Gene Names
CARHSP1; CSDC1; CRHSP24; CRHSP-24
Purity
>85% as determined by SDS-PAGE
Synonyms
CARHSP1; CARHSP1 Protein; Human; Recombinant (His Tag); Human CARHSP1 Protein (His Tag); CRHSP-24 Protein; CSDC1 Protein; calcium regulated heat stable protein 1; 24kDa; CARHSP1 recombinant protein
Host
E Coli
Purity/Purification
>85% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 50mM Tirs, 10% Glycerol, 200mM NaCl, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Ser147
Species
Human
Predicted N Terminal
His
Tag
N-His
Protein Construction
A DNA sequence encoding the human CARHSP1(Q9Y2V2)(Met1-Ser147) was expressed with a polyhistidine tag at the N-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for CARHSP1 recombinant protein
Background: CARHSP1 is a biomarker for diabetic complications. Adenovirus-mediated CARHSP1 overexpression and siRNA-mediated knockdown experiments were performed to characterize the role of CARHSP1 in the regulation of gluconeogenic gene expression. CARHSP1 is regulated by nutrient status in the liver and functions at the transcriptional level to negatively regulate gluconeogenic genes, including the glucose-6-phosphatase catalytic subunit (G6Pc) and phosphoenolpyruvate carboxykinase 1 (PEPCK1). In addition, it is found that CARHSP1 can physically interact with peroxisome proliferator-activated receptor-alpha (PPARalpha) and inhibit its transcriptional activity. Both pharmacological and genetic ablations of PPARalpha attenuate the inhibitory effect of CARHSP1 on gluconeogenic gene expression in hepatocytes.
References
Wistow G. et al., 2002, Mol Vis. 8: 205-20. Wishart MJ. et al., 2002, Proc Natl Acad Sci. 99 (4): 2112-7. Groblewski GE. et al., 1998, J Biol Chem. 273 (35): 22738-44. Fan Y. et al., 2011, J Biol Chem. 286 (47): 40584-94.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18.0 kDa (167aa), confirmed by MALDI-TOF
NCBI Official Full Name
calcium-regulated heat-stable protein 1
NCBI Official Synonym Full Names
calcium regulated heat stable protein 1
NCBI Official Symbol
CARHSP1
NCBI Official Synonym Symbols
CSDC1; CRHSP24; CRHSP-24
NCBI Protein Information
calcium-regulated heat-stable protein 1; calcium-regulated heat stable protein 1
UniProt Protein Name
Calcium-regulated heat-stable protein 1
Protein Family
UniProt Gene Name
CARHSP1
UniProt Synonym Gene Names
CRHSP-24
Uniprot Description
Binds mRNA and regulates the stability of target mRNA. Binds single-stranded DNA (in vitro).
Research Articles on CARHSP1
Similar Products
Product Notes
The CARHSP1 carhsp1 (Catalog #AAA8121672) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Ser14 7. It is sometimes possible for the material contained within the vial of "CARHSP1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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