Rabbit anti-Human CARD9 Polyclonal Antibody | anti-CARD9 antibody

CARD9 Antibody

Cat. Num. AAA1494821

• Gene Names: CARD9; CANDF2; hCARD9
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: CARD9; Polyclonal Antibody; CARD9 Antibody; hCARD9; anti-CARD9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 536

• Applicable Applications for anti-CARD9 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:500-2000; IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Caspase recruitment domain-containing protein 9 protein (237-536aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-49408 / sc-49405 / sc-99054
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: CARD9 antibody at 15ug/mlLane 1:THP-1 whole cell lysateLane 2:Jurkat whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 63,57,43 kDaObserved band size: 62 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human adrenal gland using MBS1494821 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human spleen tissue using MBS1494821 at dilution 1:100)

Related Product Information for anti-CARD9 antibody

Adapter protein that plays a key role in innate immune response to a number of intracellular pathogens, such as C.albicans and L.monocytogenes. Is at the crossroads of ITAM-tyrosine kinase and the Toll-like receptors (TLR) and NOD2 signaling pathways. Probably controls various innate immune response pathways depending on the intracellular pathogen. In response to L.monocytogenes infection, acts by connecting NOD2 recognition of peptidoglycan to downstream activation of MAP kinases (MAPK) without activating NF-kappa-B. Also involved in activation of myeloid cells via classical ITAM-associated receptors and TLR: required for TLR-mediated activation of MAPK, while it is not required for TLR-induced activation of NF-kappa-B (By similarity). Controls CLEC7A (dectin-1)-mediated myeloid cell activation induced by the yeast cell wall component zymosan, leading to cytokine production and innate anti-fungal immunity: acts by regulating BCL10-MALT1-mediated NF-kappa-B activation pathway. Activates NF-kappa-B via BCL10. In response to the hyphal form of C.albicans, mediates CLEC6A (dectin-2)-induced I-kappa-B kinase ubiquitination, leading to NF-kappa-B activation via interaction with BCL10. In response to fungal infection, may be required for the development and subsequent differentiation of interleukin 17-producing T helper (TH-17) cells.

NCBI and Uniprot Product Information

• NCBI GI #: 47717131
• NCBI GeneID: 64170
• NCBI Accession #: NP_434700.2
• NCBI GenBank Nucleotide #: NM_052813.4
• UniProt Accession #: Q9H257; Q5SXM5; Q5SXM6; Q9H854
• Molecular Weight: 42,953 Da
• NCBI Official Full Name: caspase recruitment domain-containing protein 9 isoform 1
• NCBI Official Synonym Full Names: caspase recruitment domain family member 9
• NCBI Official Symbol: CARD9
• NCBI Official Synonym Symbols: CANDF2; hCARD9
• NCBI Protein Information: caspase recruitment domain-containing protein 9
• UniProt Protein Name: Caspase recruitment domain-containing protein 9
• Protein Family: Caspase recruitment domain-containing protein
• UniProt Gene Name: CARD9
• UniProt Synonym Gene Names: hCARD9
• UniProt Entry Name: CARD9_HUMAN

NCBI Description

The protein encoded by this gene is a member of the CARD protein family, which is defined by the presence of a characteristic caspase-associated recruitment domain (CARD). CARD is a protein interaction domain known to participate in activation or suppression of CARD containing members of the caspase family, and thus plays an important regulatory role in cell apoptosis. This protein was identified by its selective association with the CARD domain of BCL10, a postive regulator of apoptosis and NF-kappaB activation, and is thought to function as a molecular scaffold for the assembly of a BCL10 signaling complex that activates NF-kappaB. Several alternatively spliced transcript variants have been observed, but their full-length nature is not clearly defined. [provided by RefSeq, Jul 2008]

Uniprot Description

CARD9: Activates NF-kappa-B via BCL10. Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2). Chronic mucocutaneous candidiasis is characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CANDF2 is an autosomal recessive chronic mucocutaneous candidiasis. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: cytoplasm; cytosol; plasma membrane

Molecular Function: CARD domain binding; protein binding; protein homodimerization activity

Biological Process: defense response to Gram-positive bacterium; defense response to virus; I-kappaB kinase/NF-kappaB cascade; innate immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-6 production; positive regulation of JNK cascade; positive regulation of stress-activated MAPK cascade; positive regulation of tumor necrosis factor production; regulation of apoptosis; regulation of interleukin-2 biosynthetic process; regulation of interleukin-6 biosynthetic process; regulation of tumor necrosis factor biosynthetic process; response to drug; response to exogenous dsRNA; response to fungus; response to muramyl dipeptide; response to peptidoglycan; stimulatory C-type lectin receptor signaling pathway

Disease: Candidiasis, Familial, 2