Western Blot (WB)
(Western blot analysis of extracts from Mouse brain tissue, using CACNA1B Antibody. The lane on the left was treated with blocking peptide.)
Rabbit CACNA1B Polyclonal Antibody | anti-CACNA1B antibody
CACNA1B Antibody
Gene Names
CACNA1B; BIII; CACNN; DYT23; Cav2.2; NEDNEH; CACNL1A5
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Peptide affinity purification
Synonyms
CACNA1B; Polyclonal Antibody; CACNA1B Antibody; BIII; Brain calcium channel III; CACH5; CACNA1B (N type); CACNL1A5; CACNN; Calcium channel alpha12.2 subunit; Calcium channel L type alpha 1 polypeptide isoform 5; Calcium channel L type; alpha 1 polypeptide; Calcium channel N type; Calcium channel voltage dependent alpha 1B subunit N type; Calcium channel voltage dependent L type alpha 1B subunit; Calcium channel voltage dependent N type alpha 1B subunit; Cav2.2; Cav2.2 voltage gated Ca2+ channel; Voltage dependent N type calcium channel subunit alpha 1B; Voltage gated calcium channel alpha subunit Cav2.2; anti-CACNA1B antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CACNA1B Antibody detects endogenous levels of CACNA1B.
Purity/Purification
Peptide affinity purification
Form/Format
Phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
2339
Applicable Applications for anti-CACNA1B antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
*The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
*The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human CACNA1B, corresponding to a region within N-terminal amino acids.
Preparation and Storage
Store at -20°C.
Western Blot (WB)
(Western blot analysis of extracts from Mouse brain tissue, using CACNA1B Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Mouse brain tissue, using CACNA1B Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-CACNA1B antibody
Description: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1B gives rise to N-type calcium currents. N-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by omega-conotoxin-GVIA (omega-CTx-GVIA) and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to dihydropyridines (DHP), and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing alpha-1B subunit may play a role in directed migration of immature neurons.
Post Translational Modifications: Phosphorylated in vitro by CaM-kinase II, PKA, PKC and CGPK.
Subunit Structure: Multisubunit complex consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. Interacts with RIMS1. Interacts with FMR1 (via C-terminus); this interaction induces a deacrease in the number of presynaptic functional CACNA1B channels at the cell surface.
Similarity: Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position. Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1B subfamily.
Post Translational Modifications: Phosphorylated in vitro by CaM-kinase II, PKA, PKC and CGPK.
Subunit Structure: Multisubunit complex consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. Interacts with RIMS1. Interacts with FMR1 (via C-terminus); this interaction induces a deacrease in the number of presynaptic functional CACNA1B channels at the cell surface.
Similarity: Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position. Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1B subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
262 kDa
NCBI Official Full Name
voltage-dependent N-type calcium channel subunit alpha-1B isoform 1
NCBI Official Synonym Full Names
calcium voltage-gated channel subunit alpha1 B
NCBI Official Symbol
CACNA1B
NCBI Official Synonym Symbols
BIII; CACNN; DYT23; Cav2.2; NEDNEH; CACNL1A5
NCBI Protein Information
voltage-dependent N-type calcium channel subunit alpha-1B
UniProt Protein Name
Voltage-dependent N-type calcium channel subunit alpha-1B
Protein Family
UniProt Gene Name
CACNA1B
UniProt Synonym Gene Names
CACH5; CACNL1A5; BIII
UniProt Entry Name
CAC1B_HUMAN
NCBI Description
The protein encoded by this gene is the pore-forming subunit of an N-type voltage-dependent calcium channel, which controls neurotransmitter release from neurons. The encoded protein forms a complex with alpha-2, beta, and delta subunits to form the high-voltage activated channel. This channel is sensitive to omega-conotoxin-GVIA and omega-agatoxin-IIIA but insensitive to dihydropyridines. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Research Articles on CACNA1B
Similar Products
Product Notes
The CACNA1B cacna1b (Catalog #AAA9611623) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CACNA1B Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CACNA1B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000 *The optimal dilutions should be determined by the end user. IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the CACNA1B cacna1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CACNA1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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