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Western Blot (WB)
(C1orf25 polyclonal antibody. Western Blot analysis of C1orf25 expression in Hela NE.)
Mouse anti-Human C1orf25 Polyclonal Antibody
C1orf25 (TRMT1-like Protein, TRMT1L, TRM1L, MSTP070, MGC25112, MGC57134)
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
C1orf25; Polyclonal Antibody; C1orf25 (TRMT1-like Protein; TRMT1L; TRM1L; MSTP070; MGC25112; MGC57134); Anti -C1orf25 (TRMT1-like Protein; anti-C1orf25 antibody
Host
Mouse
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human C1orf25.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MENMAEEELLPLEKEEVEVAQVQVPTPARDSAGVPAPAPDSALDSAPTPASAPAPAPALAQAPALSPSLASAPEEAKSKRHISIQRQLADLENLAFVTDGNFDSASSLNSDNLDAGNRQACPLCPKEKFRACNSHKLRRHLQNLHWKVSVEFEGYRMCICHLPCRPVKPNIIGEQITSKMGAHYHCIICSATITRRTDMLGHVRRHMNKGETKSSYIAASTAKPPKEILKEADTDVQVCPNYSIPQKTDSYFNPKMKLNRQLIFCTLAALAEERKPLECLDAFGATGIMGLQWAKHLGNAVKVTINDLNENSVTLIQENCHLNKLKVVVDSKEKEKSDDILEEGEKNLGNIKVTKMDANVLMHLRSFDFIHLDPFGTSVNYLDSAFRNIRNLGIVSVTSTDISSLYAKAQHVARRHYGCNIVRTEYYKELAARIVVAAVARAAARCNKGIEVLFAVALEHFVLVVVRVLRGPTSADETAKKIQYLIHCQWCEERIFQKDGNMVEENPYRQLPCNCHGSMPGKTAIELGPLWSSSLFNTGFLKRMLFESLHHGLDDIQTLIKTLIFESECTPQSQFSIHASSNVNKQEENGVFIKTTDDTTTDNYIAQGKRKSNEMITNLGKKQKTDVSTEHPPFYYNIHRHSIKGMNMPKLKKFLCYLSQAGFRVSRTHFDPMGVRTDAPLMQFKSILLKYSTPTYTGGQSESHVQSASEDTVTERVEMSVNDKAEASGCRRW
Applicable Applications for anti-C1orf25 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence, Immunohistochemistry and Western Blot.
Dilution: Immunofluorescence: 10ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml
Dilution: Immunofluorescence: 10ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml
Immunogen
Full length human C1orf25, aa1-733 (AAH45535.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(C1orf25 polyclonal antibody. Western Blot analysis of C1orf25 expression in Hela NE.)
Western Blot (WB)
(C1orf25 polyclonal antibody. Western Blot analysis of C1orf25 expression in Hela NE.)
Western Blot (WB)
(Western Blot analysis of C1orf25 expression in transfected 293T cell line by C1orf25 polyclonal antibody. Lane 1: C1orf25 transfected lysate (80.63kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of C1orf25 expression in transfected 293T cell line by C1orf25 polyclonal antibody. Lane 1: C1orf25 transfected lysate (80.63kD). Lane 2: Non-transfected lysate.)
Immunohistochemistry (IHC)
(Immunoperoxidase of purified antibody to C1orf25 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)
Immunohistochemistry (IHC)
(Immunoperoxidase of purified antibody to C1orf25 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)
Immunofluorescence (IF)
(Immunofluorescence of purified antibody to C1orf25 on HeLa cell. [antibody concentration 10ug/ml].)
Immunofluorescence (IF)
(Immunofluorescence of purified antibody to C1orf25 on HeLa cell. [antibody concentration 10ug/ml].)
Related Product Information for anti-C1orf25 antibody
May play a role in motor coordination and exploratory behavior.
Product Categories/Family for anti-C1orf25 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
C1orf25
Uniprot Description
TRM1L: May play a role in motor coordination and exploratory behavior. Belongs to the TRM1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: C2H2-type zinc finger protein; RNA-binding
Chromosomal Location of Human Ortholog: 1q25.2
Molecular Function: tRNA (guanine-N2-)-methyltransferase activity; metal ion binding; tRNA binding
Biological Process: adult locomotory behavior; tRNA methylation
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Product Notes
The C1orf25 (Catalog #AAA642822) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The C1orf25 (TRMT1-like Protein, TRMT1L, TRM1L, MSTP070, MGC25112, MGC57134) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's C1orf25 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, Immunohistochemistry and Western Blot. Dilution: Immunofluorescence: 10ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml. Researchers should empirically determine the suitability of the C1orf25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MENMAEEELL PLEKEEVEVA QVQVPTPARD SAGVPAPAPD SALDSAPTPA SAPAPAPALA QAPALSPSLA SAPEEAKSKR HISIQRQLAD LENLAFVTDG NFDSASSLNS DNLDAGNRQA CPLCPKEKFR ACNSHKLRRH LQNLHWKVSV EFEGYRMCIC HLPCRPVKPN IIGEQITSKM GAHYHCIICS ATITRRTDML GHVRRHMNKG ETKSSYIAAS TAKPPKEILK EADTDVQVCP NYSIPQKTDS YFNPKMKLNR QLIFCTLAAL AEERKPLECL DAFGATGIMG LQWAKHLGNA VKVTINDLNE NSVTLIQENC HLNKLKVVVD SKEKEKSDDI LEEGEKNLGN IKVTKMDANV LMHLRSFDFI HLDPFGTSVN YLDSAFRNIR NLGIVSVTST DISSLYAKAQ HVARRHYGCN IVRTEYYKEL AARIVVAAVA RAAARCNKGI EVLFAVALEH FVLVVVRVLR GPTSADETAK KIQYLIHCQW CEERIFQKDG NMVEENPYRQ LPCNCHGSMP GKTAIELGPL WSSSLFNTGF LKRMLFESLH HGLDDIQTLI KTLIFESECT PQSQFSIHAS SNVNKQEENG VFIKTTDDTT TDNYIAQGKR KSNEMITNLG KKQKTDVSTE HPPFYYNIHR HSIKGMNMPK LKKFLCYLSQ AGFRVSRTHF DPMGVRTDAP LMQFKSILLK YSTPTYTGGQ SESHVQSASE DTVTERVEMS VNDKAEASGC RRW. It is sometimes possible for the material contained within the vial of "C1orf25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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