Rabbit BRN3B Polyclonal Antibody | anti-BRN3B antibody

BRN3B Polyclonal Antibody

Cat. Num. AAA3015507

• Gene Names: POU4F2; BRN3B; BRN3.2; Brn-3b
• Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep
• Applications: Immunohistochemistry
• Purity: affinity purified by Protein A
• Synonyms: BRN3B; Polyclonal Antibody; BRN3B Polyclonal Antibody; class 4; transcription factor 2; Brain specific homeobox/POU domain protein 3B; Brain-3B; Brain-specific homeobox/POU domain protein 3B; Brn-3B; BRN3.2; Brn3b POU domain transcription factor; PO4F2_HUMAN; POU class 4 homeobox 2; POU domain; POU domain class 4 transcription factor 2; POU domain protein; POU4F2; anti-BRN3B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep
• Clonality: Polyclonal
• Specificity: BRN3B Polyclonal Antibody detects endogenous levels of BRN3B protein.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-BRN3B antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:100-500
• Immunogen: KLH conjugated synthetic peptide derived from human BRN3B:301-400/409
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BRN3B) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)

Related Product Information for anti-BRN3B antibody

The Brn family of transcription factors are found in a highly restricted subset of neurons and are critical to the early embryonic development of the central nervous system. Brn-1 and Brn-2 are class III POU (Pit-Oct-Unc) domain proteins, whereas Brn-3 is a class IV POU domain protein. Three Brn-3 proteins have been described and are designated Brn-3a, Brn-3b and Brn-3c. While Brn-3a and Brn-3c stimulate transcription, Brn-3b generally functions as a transcriptional repressor. However, Brn-3b, but not Brn-3a, has been shown to regulate the expression of the acetylcholine receptor. Interestingly, Brn-3a has two functional transactivating domains, one at the amino-terminus and one at the carboxy-terminus. Brn-2 is thought to be involved in smooth muscle cell development and differentiation.

NCBI and Uniprot Product Information

• NCBI GI #: 290457652
• NCBI GeneID: 5458
• UniProt Accession #: Q12837; Q13883; Q14987; B1PJR6; B2RC84
• Molecular Weight: 409
• NCBI Official Full Name: POU domain, class 4, transcription factor 2
• NCBI Official Synonym Full Names: POU class 4 homeobox 2
• NCBI Official Symbol: POU4F2
• NCBI Official Synonym Symbols: BRN3B; BRN3.2; Brn-3b
• NCBI Protein Information: POU domain, class 4, transcription factor 2; brain-3B; POU domain protein; Brn3b POU domain transcription factor; POU domain class 4 transcription factor 2; brain-specific homeobox/POU domain protein 3B
• UniProt Protein Name: POU domain, class 4, transcription factor 2
• UniProt Gene Name: POU4F2
• UniProt Synonym Gene Names: BRN3B; Brain-3B; Brn-3B
• UniProt Entry Name: PO4F2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the POU-domain transcription factor family and may be involved in maintaining visual system neurons in the retina. The level of the encoded protein is also elevated in a majority of breast cancers, resulting in accelerated tumor growth. [provided by RefSeq, Sep 2011]

Uniprot Description

POU4F2: Transcription factor. May play a role in determining or maintaining the identities of a small subset of visual system neurons. Belongs to the POU transcription factor family. Class- 4 subfamily.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 4q31.2

Cellular Component: nuclear speck; nucleus

Molecular Function: chromatin binding

Biological Process: neuron differentiation; axon guidance; estrogen receptor signaling pathway; sensory perception of sound; transcription, DNA-dependent; retina development in camera-type eye; axon extension; MAPKKK cascade; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; neuromuscular process controlling balance; positive regulation of cell differentiation; retinal ganglion cell axon guidance