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Western Blot (WB) (POU4F2 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the POU4F2 antibody detected the POU4F2 protein (arrow).)

Rabbit anti-Human POU4F2 Polyclonal Antibody | anti-POU4F2 antibody

POU4F2, CT (POU4F2, BRN3B, POU domain, class 4, transcription factor 2, Brain-specific homeobox/POU domain protein 3B) (Biotin)

Gene Names
POU4F2; BRN3B; BRN3.2; Brn-3b
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
POU4F2; Polyclonal Antibody; CT (POU4F2; BRN3B; POU domain; class 4; transcription factor 2; Brain-specific homeobox/POU domain protein 3B) (Biotin); Brain-specific homeobox/POU domain protein 3B; anti-POU4F2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-POU4F2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
POU4F2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 310-339 amino acids from the C-terminal region of human POU4F2.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(POU4F2 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the POU4F2 antibody detected the POU4F2 protein (arrow).)

Western Blot (WB) (POU4F2 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the POU4F2 antibody detected the POU4F2 protein (arrow).)
Related Product Information for anti-POU4F2 antibody
POU4F2 is a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. A class IV POU-domain protein, POU4F2 is found in human retina exclusively within a subpopulation of ganglion cells where it may play a role in determining or maintaining the identities of a small subset of visual system neurons.
Product Categories/Family for anti-POU4F2 antibody
References
Qiu, F., et al. J. Neurosci. 28(13):3392-3403(2008). Budhram-Mahadeo, V.S., et al. Oncogene 27(1):145-154(2008). Calissano, M., et al. FEBS Lett. 581(13):2490-2496(2007). Choy, K.W., et al. Physiol. Genomics 25(1):9-15(2006). Samady, L., et al. Int. J. Cancer 118(4):869-878(2006).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,087 Da
NCBI Official Full Name
POU domain, class 4, transcription factor 2
NCBI Official Synonym Full Names
POU class 4 homeobox 2
NCBI Official Symbol
POU4F2
NCBI Official Synonym Symbols
BRN3B; BRN3.2; Brn-3b
NCBI Protein Information
POU domain, class 4, transcription factor 2
UniProt Protein Name
POU domain, class 4, transcription factor 2
UniProt Gene Name
POU4F2
UniProt Synonym Gene Names
BRN3B; Brain-3B; Brn-3B
UniProt Entry Name
PO4F2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the POU-domain transcription factor family and may be involved in maintaining visual system neurons in the retina. The level of the encoded protein is also elevated in a majority of breast cancers, resulting in accelerated tumor growth. [provided by RefSeq, Sep 2011]

Uniprot Description

POU4F2: Transcription factor. May play a role in determining or maintaining the identities of a small subset of visual system neurons. Belongs to the POU transcription factor family. Class- 4 subfamily.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 4q31.2

Cellular Component: nuclear speck; nucleus

Molecular Function: chromatin binding

Biological Process: neuron differentiation; axon guidance; estrogen receptor signaling pathway; sensory perception of sound; transcription, DNA-dependent; retina development in camera-type eye; axon extension; MAPKKK cascade; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; neuromuscular process controlling balance; positive regulation of cell differentiation; retinal ganglion cell axon guidance

Research Articles on POU4F2

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Product Notes

The POU4F2 pou4f2 (Catalog #AAA6336029) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POU4F2, CT (POU4F2, BRN3B, POU domain, class 4, transcription factor 2, Brain-specific homeobox/POU domain protein 3B) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's POU4F2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the POU4F2 pou4f2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POU4F2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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