Sheep anti-Mouse FGF15 Polyclonal Antibody | anti-Fgf15 antibody

FGF15 (Fibroblast Growth Factor 15, FGF-15)

Cat. Num. AAA6012397

• Gene Names: Fgf15; FGF19
• Reactivity: Mouse
• Applications: Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: FGF15; Polyclonal Antibody; FGF15 (Fibroblast Growth Factor 15; FGF-15); Anti -FGF15 (Fibroblast Growth Factor 15; anti-Fgf15 antibody

• Host: Sheep
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse FGF-15. Species sequence homology: Recombinant human FGF-19 (10%), rhFGF-22 and rhFGF-23 (5%).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.

• Applicable Applications for anti-Fgf15 antibody: Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry.; Dilution: Immunohistochemistry: 5-15ug/ml
• Immunogen: Recombinant corresponding to aa24-218 from mouse FGF-15 expressed in E.coli.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(FGF-15 in Mouse Embryo. FGF-15 was detected in immersion fixed frozen sections of mouse embryo (E15) using MBS6012397 (15ug/ml) overnight at 4°C. Tissue was stained using the Sheep HRP-DAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). Specific staining was localized to the processes of dorsal root ganglia neurons.)

Related Product Information for anti-Fgf15 antibody

FGF-15 is a 25kD, secreted, nonglycosylated polypeptide that is a member of the FGF-15/21/23 subfamily, FGF family of growth factors. It is synthesized by ileal epithelium, colonic myofibroblasts, and select areas of the developing brain. FGF-15 acts on hepatocytes and reduces CYP7A1 levels, thus blocking bile acid production. It also promotes colonic epithelial migration, and in the embryonic brain, inhibits over-production of neuronal precursors. FGF-15 is synthesized as a 218aa precursor that contains a 25aa signal sequence and a 182aa mature region aa26-218; 23kD predicted MW). Mature FGF-15 contains a characteristic 3-dimensional B-trefoil structure, plus a unique Cys pattern that contributes to stability. There are two potential splice forms. One shows a 23aa substitution for aa120-218, while a second shows a 41aa substitution for aa120-218. Over aa23-200, mouse FGF-15 shares 96% aa identity with rat FGF-15. Although mouse FGF-15 is considered the ortholog to human FGF-19, the two molecules share only 54% aa identity over aa23-200, and are produced by different cell types (intestinal epithelium in mouse, hepatocytes in human).

Product Categories/Family for anti-Fgf15 antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 6679777
• NCBI GeneID: 14170
• NCBI Accession #: NP_032029.1
• NCBI GenBank Nucleotide #: NM_008003.2
• UniProt Accession #: O35622
• Molecular Weight: 25,236 Da
• NCBI Official Full Name: fibroblast growth factor 15
• NCBI Official Synonym Full Names: fibroblast growth factor 15
• NCBI Official Symbol: Fgf15
• NCBI Official Synonym Symbols: FGF19
• NCBI Protein Information: fibroblast growth factor 15; FGF-15
• UniProt Protein Name: Fibroblast growth factor 15
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: Fgf15
• UniProt Synonym Gene Names: FGF-15
• UniProt Entry Name: FGF15_MOUSE

Uniprot Description

FGF19: Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. Belongs to the heparin-binding growth factors family.

Protein type: Motility/polarity/chemotaxis; Secreted; Cell development/differentiation; Cytokine; Secreted, signal peptide

Cellular Component: extracellular region

Molecular Function: protein binding; growth factor activity; receptor binding; fibroblast growth factor receptor binding

Biological Process: positive regulation of glucose import; fibroblast growth factor receptor signaling pathway; heart development; positive regulation of cell proliferation; positive regulation of JNK cascade; positive regulation of protein amino acid phosphorylation; neural crest cell migration