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Western Blot (WB) (BPHL Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the BPHL antibody detected the BPHL protein (arrow).)

Rabbit anti-Human BPHL Polyclonal Antibody | anti-BPHL antibody

BPHL, ID (BPHL, MCNAA, Valacyclovir hydrolase, Biphenyl hydrolase-like protein, Biphenyl hydrolase-related protein, Breast epithelial mucin-associated antigen) (APC)

Gene Names
BPHL; MCNAA; BPH-RP; VACVASE
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
BPHL; Polyclonal Antibody; ID (BPHL; MCNAA; Valacyclovir hydrolase; Biphenyl hydrolase-like protein; Biphenyl hydrolase-related protein; Breast epithelial mucin-associated antigen) (APC); Breast epithelial mucin-associated antigen; anti-BPHL antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-BPHL antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
BPHL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-124 amino acids from the Central region of human BPHL.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(BPHL Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the BPHL antibody detected the BPHL protein (arrow).)

Western Blot (WB) (BPHL Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the BPHL antibody detected the BPHL protein (arrow).)
Related Product Information for anti-BPHL antibody
This gene encodes a member of the serine protease family of hydrolytic enzymes which contain a serine in their active site. The encoded protein may play a role in activation of the antiviral prodrug valacyclovir. Alternatively spliced transcript variants have been described.
Product Categories/Family for anti-BPHL antibody
References
Davila, S., et al. Genes Immun. 11(3):232-238(2010). Lai, L., et al. J. Biol. Chem. 283(14):9318-9327(2008). Grigo, K., et al. Biol. Chem. 389(2):179-187(2008). Melk, A., et al. Kidney Int. 68(6):2667-2679(2005). Kim, I., et al. Mol. Pharm. 1(6):434-446(2004).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
670
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31,107 Da
NCBI Official Full Name
valacyclovir hydrolase isoform 1
NCBI Official Synonym Full Names
biphenyl hydrolase-like (serine hydrolase)
NCBI Official Symbol
BPHL
NCBI Official Synonym Symbols
MCNAA; BPH-RP; VACVASE
NCBI Protein Information
valacyclovir hydrolase; biphenyl hydrolase-related protein; breast epithelial mucin-associated antigen; valacyclovirase
UniProt Protein Name
Valacyclovir hydrolase
Protein Family
UniProt Gene Name
BPHL
UniProt Synonym Gene Names
MCNAA; VACVase; Valacyclovirase; Bph-rp; MCNAA
UniProt Entry Name
BPHL_HUMAN

NCBI Description

This gene encodes a member of the serine protease family of hydrolytic enzymes which contain a serine in their active site. The encoded protein may play a role in activation of the antiviral prodrug valacyclovir. Alternatively spliced transcript variants have been described.[provided by RefSeq, Jan 2009]

Uniprot Description

BPHL: Serine hydrolase that catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. Activates valacyclovir to acyclovir. May play a role in detoxification processes. It is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol. Belongs to the AB hydrolase superfamily. Lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.1.-.-

Chromosomal Location of Human Ortholog: 6p25

Cellular Component: mitochondrion

Molecular Function: hydrolase activity

Biological Process: amino acid metabolic process; response to toxin

Research Articles on BPHL

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Product Notes

The BPHL bphl (Catalog #AAA6278759) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BPHL, ID (BPHL, MCNAA, Valacyclovir hydrolase, Biphenyl hydrolase-like protein, Biphenyl hydrolase-related protein, Breast epithelial mucin-associated antigen) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BPHL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the BPHL bphl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BPHL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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