BPHL recombinant protein

BPHL Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121506

• Gene Names: BPHL; MCNAA; BPH-RP; VACVASE
• Purity: >95% as determined by SDS-PAGE
• Synonyms: BPHL; BPHL Protein; Human; Recombinant (His Tag); Human BPHL Protein (His Tag); BPH-RP Protein; MCNAA Protein; VACVASE Protein; biphenyl hydrolase-like (serine hydrolase); BPHL recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 50mM NaCl, 10% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ser38-Gln291

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human BPHL (Q86WA6-1)(Ser38-Gln291) was expressed with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for BPHL recombinant protein

Background: BPHL is a member of the serine protease family. BPHL is expressed large quantities in liver and kidney and in minor quantities in heart, intestine and skeletal muscle. BPHL is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol. It catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. BPHL also activates valacyclovir to acyclovir. It may play a role in detoxification processes.

References

Lai L. et al., 2008, J Biol Chem. 283 (14): 9318-27. Davila S. et al., 2010, Genes Immun. 11 (3): 232-8. Hendrickson SL. et al., 2010, PLoS One. 5 (9): e12862.

NCBI and Uniprot Product Information

• NCBI GI #: 221316588
• NCBI GeneID: 670
• NCBI Accession #: NP_004323.2
• NCBI GenBank Nucleotide #: NM_004332.3
• UniProt Accession #: Q86WA6; Q00306; Q13855; Q3KP51
• Molecular Weight: 31,107 Da
• NCBI Official Full Name: valacyclovir hydrolase isoform 1
• NCBI Official Synonym Full Names: biphenyl hydrolase-like (serine hydrolase)
• NCBI Official Symbol: BPHL
• NCBI Official Synonym Symbols: MCNAA; BPH-RP; VACVASE
• NCBI Protein Information: valacyclovir hydrolase; biphenyl hydrolase-related protein; breast epithelial mucin-associated antigen; valacyclovirase
• UniProt Protein Name: Valacyclovir hydrolase
• Protein Family: Valacyclovir hydrolase
• UniProt Gene Name: BPHL
• UniProt Synonym Gene Names: MCNAA; VACVase; Valacyclovirase; Bph-rp; MCNAA
• UniProt Entry Name: BPHL_HUMAN

NCBI Description

This gene encodes a member of the serine protease family of hydrolytic enzymes which contain a serine in their active site. The encoded protein may play a role in activation of the antiviral prodrug valacyclovir. Alternatively spliced transcript variants have been described.[provided by RefSeq, Jan 2009]

Uniprot Description

BPHL: Serine hydrolase that catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. Activates valacyclovir to acyclovir. May play a role in detoxification processes. It is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol. Belongs to the AB hydrolase superfamily. Lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.1.-.-

Chromosomal Location of Human Ortholog: 6p25

Cellular Component: mitochondrion

Molecular Function: hydrolase activity

Biological Process: amino acid metabolic process; response to toxin