Rabbit anti-Human, Mouse BMI-1 Polyclonal Antibody | anti-BMI-1 antibody

BMI-1, CT (Polycomb Complex Protein BMI-1, Polycomb Group RING Finger Protein 4, RING Finger Protein 51, BMI1, PCGF4, RNF51) (APC)

Cat. Num. AAA6465429

• Gene Names: BMI1; PCGF4; RNF51; FLVI2/BMI1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: BMI-1; Polyclonal Antibody; CT (Polycomb Complex Protein BMI-1; Polycomb Group RING Finger Protein 4; RING Finger Protein 51; BMI1; PCGF4; RNF51) (APC); anti-BMI-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BMI. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-BMI-1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human BMI (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of BMI1 Antibody (C-term) antibody in 293 cell line lysates (35ug/lane). BMI1(arrow) was detected using the purified Pab.)

Western Blot (WB)

(BMI1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the BMI1 antibody detected the BMI1 protein (arrow).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with BMI1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-BMI-1 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

1. Smith, K.S., et al., Mol. Cell 12(2):393-400 (2003). 2. Xia, Z.B., et al., Proc. Natl. Acad. Sci. USA 100(14):8342-8347 (2003). 3. Itahana, K., et al., Mol. Cell. Biol. 23(1):389-401 (2003). 4. Dimri, G.P., et al., Cancer Res. 62(16):4736-4745 (2002). 5. Bardos, J.I., et al., J. Biol. Chem. 275(37):28785-28792 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 27883842
• NCBI GeneID: 648
• NCBI Accession #: NP_005171
• NCBI GenBank Nucleotide #: NM_005180
• UniProt Accession #: P35226; Q16030; Q5T8Z3; Q96F37
• Molecular Weight: 36,949 Da
• NCBI Official Full Name: polycomb complex protein BMI-1
• NCBI Official Synonym Full Names: BMI1 proto-oncogene, polycomb ring finger
• NCBI Official Symbol: BMI1
• NCBI Official Synonym Symbols: PCGF4; RNF51; FLVI2/BMI1
• NCBI Protein Information: polycomb complex protein BMI-1; B lymphoma Mo-MLV insertion region 1 homolog; BMI1 polycomb ring finger oncogene; BMI1 polycomb ring finger proto-oncogene; flvi-2/bmi-1; murine leukemia viral (bmi-1) oncogene homolog; polycomb group RING finger protein 4
• UniProt Protein Name: Polycomb complex protein BMI-1
• UniProt Gene Name: BMI1
• UniProt Synonym Gene Names: PCGF4; RNF51
• UniProt Entry Name: BMI1_HUMAN

Uniprot Description

BMI1: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. Component of a PRC1-like complex. Interacts with RING1 and RING2. Interacts vwith CBX7 and CBX8. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1.

Protein type: Motility/polarity/chemotaxis; Oncoprotein; Ligase; EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 10p11.23

Cellular Component: nucleoplasm; nuclear body; heterochromatin; cytoplasm; PcG protein complex; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; zinc ion binding; sequence-specific DNA binding; ubiquitin-protein ligase activity; chromatin binding

Biological Process: transcription, DNA-dependent; in utero embryonic development; positive regulation of immature T cell proliferation in the thymus; negative regulation of transcription from RNA polymerase II promoter; humoral immune response; embryonic skeletal morphogenesis; segment specification; rostrocaudal neural tube patterning; positive regulation of fibroblast proliferation; regulation of gene expression; positive regulation of ubiquitin-protein ligase activity; histone ubiquitination; DNA methylation; positive regulation of B cell proliferation; somatic stem cell division; hemopoiesis; brain development; histone acetylation