FLRG / Fstl3 recombinant protein

Recombinant Mouse FLRG / Fstl3 Protein (His tag)

Cat. Num. AAA2547200

• Gene Names: Fstl3; Flrg; E030038F23Rik
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: FLRG / Fstl3; Recombinant Mouse FLRG / Fstl3 Protein (His tag); Fstl3; E030038F23Rik; Flrg; FLRG / Fstl3 recombinant protein

• Host: Human Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 256

• Application Notes: The secreted recombinant mouse FLRG generated after removal of the signal peptide comprises 244 amino acids with a predicted molecular mass of 26 kDa. As a result of glycosylation, it migrates as an approximately 35-40 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Val 24
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for FLRG / Fstl3 recombinant protein

Background: Follistatin-like 3 (FLRG/Fstl3) is a secreted glycoprotein of the follistatin-module-protein family. It may have a role in leukemogenesis. FLRG/Fstl3 is a recently described member of the FST family having an overall structure and activity profile similar to that of FST, including binding and neutralization of activin. FLRG/Fstl3 is expressed in a wide range of adult tissues, not detected in hematopoietic cells except in patients with a B cell chronic leukemia and a translocation. Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiationc. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 of FLRG/Fstl3 or the nuclear form of FLRG/Fstl3 is probably involved in transcriptional regulation via interaction with MLLT10. Modulation of activin and other TGFbeta superfamily signaling is the primary mechanism of action for both follistatin (FS) and FS-like 3 (FSTL-3). FLRG/Fstl3 is likely to be a local regulator of activin action in gonadal development and gametogenesis and, further, that activin appears to have important actions in gonadal development and function that are critical for normal reproduction.

Description: A DNA sequence encoding the mouse FLRG (NP_113557.1) (Met 1-Val 256) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 13878203
• NCBI GeneID: 83554
• NCBI Accession #: NP_113557.1
• NCBI GenBank Nucleotide #: NM_031380.2
• UniProt Accession #: Q9EQC7
• Molecular Weight: 27,271 Da
• NCBI Official Full Name: follistatin-related protein 3
• NCBI Official Synonym Full Names: follistatin-like 3
• NCBI Official Symbol: Fstl3
• NCBI Official Synonym Symbols: Flrg; E030038F23Rik
• NCBI Protein Information: follistatin-related protein 3
• UniProt Protein Name: Follistatin-related protein 3
• UniProt Gene Name: Fstl3
• UniProt Synonym Gene Names: Flrg

Uniprot Description

FSTL3: Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiationc. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10. A chromosomal aberration involving FSTL3 is found in a case of B-cell chronic lymphocytic leukemia. Translocation t(11;19)(q13;p13) with CCDN1. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Oncoprotein; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10|10 C1

Cellular Component: extracellular space; Golgi apparatus; nucleoplasm; nucleus; secretory granule

Molecular Function: activin binding; fibronectin binding

Biological Process: hemopoietic progenitor cell differentiation; negative regulation of activin receptor signaling pathway; negative regulation of BMP signaling pathway; negative regulation of osteoclast differentiation; positive regulation of cell-cell adhesion; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter